Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03080 and SGL_RS19410 are separated by 20 nucleotides SGL_RS19410 and SGL_RS03085 are separated by 60 nucleotides SGL_RS03085 and SGL_RS03090 are separated by 116 nucleotides SGL_RS03090 and SGL_RS03095 are separated by 55 nucleotides SGL_RS03095 and SGL_RS03100 are separated by 224 nucleotides
SGL_RS03080: SGL_RS03080 - transaldolase, at 260,463 to 261,638
_RS03080
SGL_RS19410: SGL_RS19410 - hypothetical protein, at 261,659 to 261,802
_RS19410
SGL_RS03085: SGL_RS03085 - DUF2862 domain-containing protein, at 261,863 to 262,060
_RS03085
SGL_RS03090: SGL_RS03090 - ArsA family ATPase, at 262,177 to 263,265
_RS03090
SGL_RS03095: SGL_RS03095 - peptide-methionine (S)-S-oxide reductase MsrA, at 263,321 to 263,965
_RS03095
SGL_RS03100: SGL_RS03100 - DUF2288 domain-containing protein, at 264,190 to 264,477
_RS03100
Position (kb) 262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 261.192 kb on - strand, within SGL_RS03080 at 261.265 kb on + strand, within SGL_RS03080 at 261.276 kb on + strand, within SGL_RS03080 at 261.278 kb on - strand, within SGL_RS03080 at 261.310 kb on - strand, within SGL_RS03080 at 261.310 kb on - strand, within SGL_RS03080 at 261.395 kb on - strand, within SGL_RS03080 at 261.447 kb on - strand, within SGL_RS03080 at 261.518 kb on + strand, within SGL_RS03080 at 261.526 kb on - strand at 261.537 kb on - strand at 261.703 kb on - strand, within SGL_RS19410 at 261.743 kb on + strand, within SGL_RS19410 at 261.748 kb on + strand, within SGL_RS19410 at 261.876 kb on - strand at 262.005 kb on + strand, within SGL_RS03085 at 262.013 kb on - strand, within SGL_RS03085 at 262.067 kb on + strand at 262.219 kb on - strand at 262.269 kb on + strand at 262.271 kb on + strand at 262.272 kb on - strand at 262.282 kb on - strand at 262.315 kb on + strand, within SGL_RS03090 at 262.322 kb on - strand, within SGL_RS03090 at 262.324 kb on - strand, within SGL_RS03090 at 262.336 kb on + strand, within SGL_RS03090 at 262.384 kb on - strand, within SGL_RS03090 at 262.392 kb on + strand, within SGL_RS03090 at 262.478 kb on - strand, within SGL_RS03090 at 262.509 kb on + strand, within SGL_RS03090 at 262.576 kb on - strand, within SGL_RS03090 at 262.586 kb on - strand, within SGL_RS03090 at 262.666 kb on + strand, within SGL_RS03090 at 262.727 kb on - strand, within SGL_RS03090 at 262.781 kb on + strand, within SGL_RS03090 at 262.877 kb on + strand, within SGL_RS03090 at 263.008 kb on + strand, within SGL_RS03090 at 263.025 kb on + strand, within SGL_RS03090 at 263.025 kb on + strand, within SGL_RS03090 at 263.129 kb on + strand, within SGL_RS03090 at 263.216 kb on - strand at 263.265 kb on + strand at 263.317 kb on - strand at 263.317 kb on - strand at 263.501 kb on - strand, within SGL_RS03095 at 263.551 kb on - strand, within SGL_RS03095 at 263.685 kb on + strand, within SGL_RS03095 at 263.685 kb on + strand, within SGL_RS03095 at 263.730 kb on + strand, within SGL_RS03095 at 263.738 kb on - strand, within SGL_RS03095 at 263.763 kb on - strand, within SGL_RS03095 at 263.788 kb on - strand, within SGL_RS03095 at 263.806 kb on - strand, within SGL_RS03095 at 263.859 kb on + strand, within SGL_RS03095 at 264.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 261,192 - SGL_RS03080 0.62 -0.1 261,265 + SGL_RS03080 0.68 -0.0 261,276 + SGL_RS03080 0.69 +0.3 261,278 - SGL_RS03080 0.69 -0.1 261,310 - SGL_RS03080 0.72 +0.2 261,310 - SGL_RS03080 0.72 -0.1 261,395 - SGL_RS03080 0.79 -0.2 261,447 - SGL_RS03080 0.84 +0.3 261,518 + SGL_RS03080 0.90 -0.0 261,526 - -0.2 261,537 - +0.4 261,703 - SGL_RS19410 0.31 -0.2 261,743 + SGL_RS19410 0.58 -0.2 261,748 + SGL_RS19410 0.62 +0.3 261,876 - -2.9 262,005 + SGL_RS03085 0.72 -2.3 262,013 - SGL_RS03085 0.76 -2.8 262,067 + -0.2 262,219 - -0.4 262,269 + -0.8 262,271 + -0.7 262,272 - -0.9 262,282 - -0.6 262,315 + SGL_RS03090 0.13 -0.7 262,322 - SGL_RS03090 0.13 -1.0 262,324 - SGL_RS03090 0.13 +0.0 262,336 + SGL_RS03090 0.15 -0.6 262,384 - SGL_RS03090 0.19 -0.8 262,392 + SGL_RS03090 0.20 -0.6 262,478 - SGL_RS03090 0.28 -0.4 262,509 + SGL_RS03090 0.30 -0.9 262,576 - SGL_RS03090 0.37 -1.5 262,586 - SGL_RS03090 0.38 +0.2 262,666 + SGL_RS03090 0.45 -0.9 262,727 - SGL_RS03090 0.51 -0.9 262,781 + SGL_RS03090 0.55 -0.0 262,877 + SGL_RS03090 0.64 -0.9 263,008 + SGL_RS03090 0.76 -0.9 263,025 + SGL_RS03090 0.78 -1.2 263,025 + SGL_RS03090 0.78 -0.8 263,129 + SGL_RS03090 0.87 -1.7 263,216 - -0.8 263,265 + +0.4 263,317 - -0.3 263,317 - -0.0 263,501 - SGL_RS03095 0.28 -0.1 263,551 - SGL_RS03095 0.36 +0.3 263,685 + SGL_RS03095 0.56 -0.1 263,685 + SGL_RS03095 0.56 -0.3 263,730 + SGL_RS03095 0.63 -0.1 263,738 - SGL_RS03095 0.65 +0.4 263,763 - SGL_RS03095 0.69 +0.9 263,788 - SGL_RS03095 0.72 +0.2 263,806 - SGL_RS03095 0.75 +0.3 263,859 + SGL_RS03095 0.83 -0.1 264,026 - +0.0
Or see this region's nucleotide sequence