Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11085 and SGL_RS20205 are separated by 275 nucleotides SGL_RS20205 and SGL_RS11090 are separated by 240 nucleotides SGL_RS11090 and SGL_RS11095 are separated by 52 nucleotides
SGL_RS11085: SGL_RS11085 - glucosylglycerol-phosphate synthase, at 1,947,325 to 1,948,824
_RS11085
SGL_RS20205: SGL_RS20205 - glucosylglycerol biosynthesis transcriptional repressor GgpR, at 1,949,100 to 1,949,372
_RS20205
SGL_RS11090: SGL_RS11090 - glucosylglycerol hydrolase, at 1,949,613 to 1,952,270
_RS11090
SGL_RS11095: SGL_RS11095 - glycerol kinase GlpK, at 1,952,323 to 1,953,810
_RS11095
Position (kb)
1949
1950
1951
1952
1953 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1948.797 kb on + strand at 1948.805 kb on - strand at 1949.128 kb on - strand, within SGL_RS20205 at 1949.536 kb on + strand at 1949.536 kb on + strand at 1949.554 kb on - strand at 1949.565 kb on - strand at 1949.838 kb on - strand at 1949.840 kb on + strand at 1949.943 kb on + strand, within SGL_RS11090 at 1950.053 kb on - strand, within SGL_RS11090 at 1950.087 kb on + strand, within SGL_RS11090 at 1950.203 kb on + strand, within SGL_RS11090 at 1950.203 kb on + strand, within SGL_RS11090 at 1950.203 kb on + strand, within SGL_RS11090 at 1950.203 kb on + strand, within SGL_RS11090 at 1950.203 kb on + strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.211 kb on - strand, within SGL_RS11090 at 1950.225 kb on + strand, within SGL_RS11090 at 1950.285 kb on + strand, within SGL_RS11090 at 1950.366 kb on - strand, within SGL_RS11090 at 1950.416 kb on + strand, within SGL_RS11090 at 1950.422 kb on - strand, within SGL_RS11090 at 1950.422 kb on - strand, within SGL_RS11090 at 1950.424 kb on - strand, within SGL_RS11090 at 1950.424 kb on - strand, within SGL_RS11090 at 1950.608 kb on + strand, within SGL_RS11090 at 1950.848 kb on + strand, within SGL_RS11090 at 1950.936 kb on - strand, within SGL_RS11090 at 1951.034 kb on - strand, within SGL_RS11090 at 1951.120 kb on + strand, within SGL_RS11090 at 1951.249 kb on - strand, within SGL_RS11090 at 1951.379 kb on - strand, within SGL_RS11090 at 1951.390 kb on + strand, within SGL_RS11090 at 1951.456 kb on - strand, within SGL_RS11090 at 1951.458 kb on + strand, within SGL_RS11090 at 1951.472 kb on + strand, within SGL_RS11090 at 1951.557 kb on - strand, within SGL_RS11090 at 1951.569 kb on + strand, within SGL_RS11090 at 1951.589 kb on - strand, within SGL_RS11090 at 1951.595 kb on - strand, within SGL_RS11090 at 1951.687 kb on - strand, within SGL_RS11090 at 1951.749 kb on + strand, within SGL_RS11090 at 1951.905 kb on + strand, within SGL_RS11090 at 1951.955 kb on - strand, within SGL_RS11090 at 1951.960 kb on + strand, within SGL_RS11090 at 1951.986 kb on - strand, within SGL_RS11090 at 1952.021 kb on + strand at 1952.041 kb on - strand at 1952.283 kb on - strand at 1952.319 kb on + strand at 1952.361 kb on - strand at 1952.437 kb on + strand at 1952.450 kb on - strand at 1952.469 kb on + strand at 1952.598 kb on + strand, within SGL_RS11095 at 1952.605 kb on - strand, within SGL_RS11095 at 1952.844 kb on - strand, within SGL_RS11095 at 1952.861 kb on - strand, within SGL_RS11095 at 1952.880 kb on - strand, within SGL_RS11095 at 1952.899 kb on - strand, within SGL_RS11095 at 1953.158 kb on + strand, within SGL_RS11095
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,948,797 + -5.1 1,948,805 - -5.0 1,949,128 - SGL_RS20205 0.10 -1.1 1,949,536 + +0.2 1,949,536 + +0.4 1,949,554 - -3.4 1,949,565 - -4.5 1,949,838 - -3.3 1,949,840 + -3.0 1,949,943 + SGL_RS11090 0.12 -2.1 1,950,053 - SGL_RS11090 0.17 -5.0 1,950,087 + SGL_RS11090 0.18 -4.5 1,950,203 + SGL_RS11090 0.22 -5.5 1,950,203 + SGL_RS11090 0.22 -3.9 1,950,203 + SGL_RS11090 0.22 -4.2 1,950,203 + SGL_RS11090 0.22 -4.1 1,950,203 + SGL_RS11090 0.22 -2.3 1,950,211 - SGL_RS11090 0.22 -3.7 1,950,211 - SGL_RS11090 0.22 -6.0 1,950,211 - SGL_RS11090 0.22 -4.8 1,950,211 - SGL_RS11090 0.22 -2.6 1,950,211 - SGL_RS11090 0.22 -5.3 1,950,211 - SGL_RS11090 0.22 -3.5 1,950,225 + SGL_RS11090 0.23 -3.8 1,950,285 + SGL_RS11090 0.25 -3.6 1,950,366 - SGL_RS11090 0.28 -3.8 1,950,416 + SGL_RS11090 0.30 -4.7 1,950,422 - SGL_RS11090 0.30 -5.4 1,950,422 - SGL_RS11090 0.30 -5.8 1,950,424 - SGL_RS11090 0.31 -1.6 1,950,424 - SGL_RS11090 0.31 -4.1 1,950,608 + SGL_RS11090 0.37 -4.2 1,950,848 + SGL_RS11090 0.46 -4.0 1,950,936 - SGL_RS11090 0.50 -6.0 1,951,034 - SGL_RS11090 0.53 -2.3 1,951,120 + SGL_RS11090 0.57 -3.4 1,951,249 - SGL_RS11090 0.62 -4.1 1,951,379 - SGL_RS11090 0.66 -3.6 1,951,390 + SGL_RS11090 0.67 -5.3 1,951,456 - SGL_RS11090 0.69 -4.8 1,951,458 + SGL_RS11090 0.69 -4.3 1,951,472 + SGL_RS11090 0.70 -2.5 1,951,557 - SGL_RS11090 0.73 -2.2 1,951,569 + SGL_RS11090 0.74 -2.9 1,951,589 - SGL_RS11090 0.74 -5.8 1,951,595 - SGL_RS11090 0.75 -3.9 1,951,687 - SGL_RS11090 0.78 -1.1 1,951,749 + SGL_RS11090 0.80 -5.8 1,951,905 + SGL_RS11090 0.86 -2.5 1,951,955 - SGL_RS11090 0.88 -1.8 1,951,960 + SGL_RS11090 0.88 -1.6 1,951,986 - SGL_RS11090 0.89 -3.8 1,952,021 + -3.4 1,952,041 - -3.1 1,952,283 - -0.1 1,952,319 + -2.9 1,952,361 - +0.0 1,952,437 + +0.7 1,952,450 - +0.2 1,952,469 + +0.5 1,952,598 + SGL_RS11095 0.18 -0.2 1,952,605 - SGL_RS11095 0.19 -1.1 1,952,844 - SGL_RS11095 0.35 +0.5 1,952,861 - SGL_RS11095 0.36 -0.4 1,952,880 - SGL_RS11095 0.37 +0.0 1,952,899 - SGL_RS11095 0.39 -0.4 1,953,158 + SGL_RS11095 0.56 +0.4
Or see this region's nucleotide sequence