Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02360 and SGL_RS02365 are separated by 130 nucleotides SGL_RS02365 and SGL_RS02370 are separated by 249 nucleotides SGL_RS02370 and SGL_RS02375 overlap by 4 nucleotides
SGL_RS02360: SGL_RS02360 - O-antigen ligase, at 112,860 to 114,188
_RS02360
SGL_RS02365: SGL_RS02365 - DUF3181 family protein, at 114,319 to 114,624
_RS02365
SGL_RS02370: SGL_RS02370 - DUF3119 family protein, at 114,874 to 115,272
_RS02370
SGL_RS02375: SGL_RS02375 - DUF3086 domain-containing protein, at 115,269 to 116,477
_RS02375
Position (kb) 114
115 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 113.380 kb on + strand, within SGL_RS02360 at 114.339 kb on - strand at 114.494 kb on - strand, within SGL_RS02365 at 114.713 kb on - strand at 114.788 kb on + strand at 114.856 kb on + strand at 114.856 kb on + strand at 114.884 kb on + strand at 114.978 kb on + strand, within SGL_RS02370 at 115.049 kb on + strand, within SGL_RS02370 at 115.415 kb on + strand, within SGL_RS02375 at 115.512 kb on + strand, within SGL_RS02375 at 115.586 kb on + strand, within SGL_RS02375 at 115.586 kb on + strand, within SGL_RS02375
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 113,380 + SGL_RS02360 0.39 +0.1 114,339 - -0.2 114,494 - SGL_RS02365 0.57 +0.3 114,713 - -1.4 114,788 + +0.6 114,856 + -0.4 114,856 + +0.6 114,884 + -1.3 114,978 + SGL_RS02370 0.26 -0.1 115,049 + SGL_RS02370 0.44 -0.1 115,415 + SGL_RS02375 0.12 -5.4 115,512 + SGL_RS02375 0.20 -4.1 115,586 + SGL_RS02375 0.26 -3.8 115,586 + SGL_RS02375 0.26 -1.1
Or see this region's nucleotide sequence