Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16835 and SGL_RS16840 are separated by 22 nucleotides SGL_RS16840 and SGL_RS16845 overlap by 4 nucleotides SGL_RS16845 and SGL_RS16850 are separated by 6 nucleotides
SGL_RS16835: SGL_RS16835 - ATP-binding protein, at 3,231,569 to 3,232,879
_RS16835
SGL_RS16840: SGL_RS16840 - TMEM165/GDT1 family protein, at 3,232,902 to 3,233,180
_RS16840
SGL_RS16845: SGL_RS16845 - ATP phosphoribosyltransferase, at 3,233,177 to 3,233,809
_RS16845
SGL_RS16850: SGL_RS16850 - bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU, at 3,233,816 to 3,235,186
_RS16850
Position (kb) 3232
3233
3234 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3232.000 kb on + strand, within SGL_RS16835 at 3232.391 kb on - strand, within SGL_RS16835 at 3232.458 kb on + strand, within SGL_RS16835 at 3232.619 kb on + strand, within SGL_RS16835 at 3232.719 kb on - strand, within SGL_RS16835 at 3232.869 kb on - strand at 3232.869 kb on - strand at 3233.082 kb on - strand, within SGL_RS16840 at 3233.618 kb on - strand, within SGL_RS16845
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 3,232,000 + SGL_RS16835 0.33 -0.5 3,232,391 - SGL_RS16835 0.63 -0.9 3,232,458 + SGL_RS16835 0.68 +0.4 3,232,619 + SGL_RS16835 0.80 -0.1 3,232,719 - SGL_RS16835 0.88 -1.0 3,232,869 - -0.7 3,232,869 - -0.1 3,233,082 - SGL_RS16840 0.65 -5.4 3,233,618 - SGL_RS16845 0.70 -0.0
Or see this region's nucleotide sequence