Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16805 and SGL_RS16810 are separated by 100 nucleotides SGL_RS16810 and SGL_RS16815 are separated by 87 nucleotides SGL_RS16815 and SGL_RS16820 are separated by 18 nucleotides
SGL_RS16805: SGL_RS16805 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 3,227,379 to 3,227,909
_RS16805
SGL_RS16810: SGL_RS16810 - single-stranded DNA-binding protein, at 3,228,010 to 3,228,375
_RS16810
SGL_RS16815: SGL_RS16815 - hypothetical protein, at 3,228,463 to 3,228,693
_RS16815
SGL_RS16820: SGL_RS16820 - 4-hydroxybenzoate solanesyltransferase, at 3,228,712 to 3,229,590
_RS16820
Position (kb) 3228
3229 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 3227.474 kb on + strand, within SGL_RS16805 at 3227.627 kb on - strand, within SGL_RS16805 at 3227.850 kb on + strand, within SGL_RS16805 at 3228.441 kb on + strand at 3228.482 kb on + strand at 3228.489 kb on + strand, within SGL_RS16815 at 3228.489 kb on + strand, within SGL_RS16815 at 3228.552 kb on - strand, within SGL_RS16815 at 3228.553 kb on + strand, within SGL_RS16815 at 3228.561 kb on - strand, within SGL_RS16815 at 3228.594 kb on + strand, within SGL_RS16815 at 3228.659 kb on - strand, within SGL_RS16815 at 3229.032 kb on - strand, within SGL_RS16820 at 3229.107 kb on + strand, within SGL_RS16820 at 3229.281 kb on - strand, within SGL_RS16820 at 3229.442 kb on + strand, within SGL_RS16820 at 3229.573 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 3,227,474 + SGL_RS16805 0.18 +0.4 3,227,627 - SGL_RS16805 0.47 -1.0 3,227,850 + SGL_RS16805 0.89 -0.2 3,228,441 + -0.8 3,228,482 + -5.9 3,228,489 + SGL_RS16815 0.11 -3.9 3,228,489 + SGL_RS16815 0.11 -4.8 3,228,552 - SGL_RS16815 0.39 -2.8 3,228,553 + SGL_RS16815 0.39 -6.1 3,228,561 - SGL_RS16815 0.42 -4.6 3,228,594 + SGL_RS16815 0.57 -5.5 3,228,659 - SGL_RS16815 0.85 -3.6 3,229,032 - SGL_RS16820 0.36 -1.3 3,229,107 + SGL_RS16820 0.45 +0.4 3,229,281 - SGL_RS16820 0.65 +1.0 3,229,442 + SGL_RS16820 0.83 -0.0 3,229,573 + -5.2
Or see this region's nucleotide sequence