Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14465 and SGL_RS14470 are separated by 116 nucleotides SGL_RS14470 and SGL_RS14475 are separated by 213 nucleotides SGL_RS14475 and SGL_RS14480 are separated by 125 nucleotides
SGL_RS14465: SGL_RS14465 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 2,698,258 to 2,699,145
_RS14465
SGL_RS14470: SGL_RS14470 - hypothetical protein, at 2,699,262 to 2,699,855
_RS14470
SGL_RS14475: SGL_RS14475 - alpha/beta hydrolase, at 2,700,069 to 2,700,818
_RS14475
SGL_RS14480: SGL_RS14480 - uroporphyrinogen-III C-methyltransferase, at 2,700,944 to 2,701,735
_RS14480
Position (kb)
2700
2701 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2699.156 kb on + strand at 2699.172 kb on - strand at 2699.214 kb on + strand at 2699.286 kb on - strand at 2699.287 kb on + strand at 2699.295 kb on - strand at 2699.350 kb on - strand, within SGL_RS14470 at 2699.357 kb on + strand, within SGL_RS14470 at 2699.630 kb on + strand, within SGL_RS14470 at 2699.633 kb on - strand, within SGL_RS14470 at 2699.742 kb on - strand, within SGL_RS14470 at 2700.103 kb on + strand at 2700.189 kb on - strand, within SGL_RS14475 at 2700.241 kb on + strand, within SGL_RS14475 at 2700.596 kb on + strand, within SGL_RS14475 at 2700.596 kb on + strand, within SGL_RS14475 at 2700.807 kb on - strand at 2700.956 kb on + strand at 2701.126 kb on - strand, within SGL_RS14480 at 2701.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,699,156 + +0.4 2,699,172 - +0.2 2,699,214 + -1.9 2,699,286 - +1.0 2,699,287 + +2.3 2,699,295 - -0.1 2,699,350 - SGL_RS14470 0.15 -0.5 2,699,357 + SGL_RS14470 0.16 -0.5 2,699,630 + SGL_RS14470 0.62 -1.8 2,699,633 - SGL_RS14470 0.62 -1.3 2,699,742 - SGL_RS14470 0.81 -1.0 2,700,103 + -0.3 2,700,189 - SGL_RS14475 0.16 -0.2 2,700,241 + SGL_RS14475 0.23 -2.1 2,700,596 + SGL_RS14475 0.70 -2.1 2,700,596 + SGL_RS14475 0.70 -1.1 2,700,807 - -0.0 2,700,956 + -1.9 2,701,126 - SGL_RS14480 0.23 +0.3 2,701,729 + -2.9
Or see this region's nucleotide sequence