Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12345 and SGL_RS12350 are separated by 123 nucleotides SGL_RS12350 and SGL_RS12355 are separated by 0 nucleotides SGL_RS12355 and SGL_RS12360 overlap by 26 nucleotides
SGL_RS12345: SGL_RS12345 - pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha, at 2,230,487 to 2,231,515
_RS12345
SGL_RS12350: SGL_RS12350 - MFS transporter, at 2,231,639 to 2,233,036
_RS12350
SGL_RS12355: SGL_RS12355 - exodeoxyribonuclease III, at 2,233,037 to 2,233,834
_RS12355
SGL_RS12360: SGL_RS12360 - DUF3750 domain-containing protein, at 2,233,809 to 2,234,273
_RS12360
Position (kb)
2231
2232
2233
2234 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2231.545 kb on + strand at 2231.648 kb on + strand at 2231.715 kb on - strand at 2232.389 kb on + strand, within SGL_RS12350 at 2232.396 kb on + strand, within SGL_RS12350 at 2232.508 kb on - strand, within SGL_RS12350 at 2232.543 kb on + strand, within SGL_RS12350 at 2232.693 kb on + strand, within SGL_RS12350 at 2232.722 kb on - strand, within SGL_RS12350 at 2232.920 kb on + strand at 2233.372 kb on + strand, within SGL_RS12355 at 2233.417 kb on - strand, within SGL_RS12355 at 2233.475 kb on + strand, within SGL_RS12355 at 2233.475 kb on + strand, within SGL_RS12355 at 2233.529 kb on - strand, within SGL_RS12355 at 2233.564 kb on - strand, within SGL_RS12355 at 2233.646 kb on + strand, within SGL_RS12355 at 2233.868 kb on + strand, within SGL_RS12360 at 2233.869 kb on - strand, within SGL_RS12360 at 2233.969 kb on + strand, within SGL_RS12360
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,231,545 + -1.9 2,231,648 + -2.2 2,231,715 - -5.8 2,232,389 + SGL_RS12350 0.54 -5.7 2,232,396 + SGL_RS12350 0.54 -5.1 2,232,508 - SGL_RS12350 0.62 -4.8 2,232,543 + SGL_RS12350 0.65 -3.3 2,232,693 + SGL_RS12350 0.75 -4.7 2,232,722 - SGL_RS12350 0.77 -5.2 2,232,920 + -4.7 2,233,372 + SGL_RS12355 0.42 -0.1 2,233,417 - SGL_RS12355 0.48 +0.4 2,233,475 + SGL_RS12355 0.55 +1.3 2,233,475 + SGL_RS12355 0.55 +0.8 2,233,529 - SGL_RS12355 0.62 -0.3 2,233,564 - SGL_RS12355 0.66 +2.0 2,233,646 + SGL_RS12355 0.76 +1.0 2,233,868 + SGL_RS12360 0.13 +1.6 2,233,869 - SGL_RS12360 0.13 +0.7 2,233,969 + SGL_RS12360 0.34 +1.8
Or see this region's nucleotide sequence