Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09480 and SGL_RS09485 are separated by 105 nucleotides SGL_RS09485 and SGL_RS09490 overlap by 11 nucleotides SGL_RS09490 and SGL_RS19865 are separated by 69 nucleotides SGL_RS19865 and SGL_RS18860 overlap by 91 nucleotides SGL_RS18860 and SGL_RS09505 overlap by 32 nucleotides
SGL_RS09480: SGL_RS09480 - IS4-like element IS4Sa family transposase, at 1,624,643 to 1,625,095
_RS09480
SGL_RS09485: SGL_RS09485 - sugar phosphate isomerase/epimerase, at 1,625,201 to 1,625,632
_RS09485
SGL_RS09490: SGL_RS09490 - CBS domain-containing protein, at 1,625,622 to 1,626,095
_RS09490
SGL_RS19865: SGL_RS19865 - IS630 transposase-related protein, at 1,626,165 to 1,626,524
_RS19865
SGL_RS18860: SGL_RS18860 - IS630 family transposase, at 1,626,434 to 1,627,012
_RS18860
SGL_RS09505: SGL_RS09505 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,626,981 to 1,628,018
_RS09505
Position (kb)
1625
1626
1627 Strain fitness (log2 ratio)
-1
0
1
2 at 1624.666 kb on - strand at 1625.182 kb on + strand at 1625.182 kb on + strand at 1625.318 kb on + strand, within SGL_RS09485 at 1625.889 kb on + strand, within SGL_RS09490 at 1625.897 kb on - strand, within SGL_RS09490 at 1625.907 kb on + strand, within SGL_RS09490 at 1626.001 kb on - strand, within SGL_RS09490 at 1626.001 kb on - strand, within SGL_RS09490
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,624,666 - +2.0 1,625,182 + -0.1 1,625,182 + +0.0 1,625,318 + SGL_RS09485 0.27 -0.8 1,625,889 + SGL_RS09490 0.56 -0.6 1,625,897 - SGL_RS09490 0.58 +0.5 1,625,907 + SGL_RS09490 0.60 +1.4 1,626,001 - SGL_RS09490 0.80 +1.3 1,626,001 - SGL_RS09490 0.80 +0.9
Or see this region's nucleotide sequence