Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05030 and SGL_RS05035 are separated by 271 nucleotides SGL_RS05035 and SGL_RS05040 are separated by 179 nucleotides SGL_RS05040 and SGL_RS05050 are separated by 218 nucleotides
SGL_RS05030: SGL_RS05030 - dienelactone hydrolase family protein, at 678,283 to 679,023
_RS05030
SGL_RS05035: SGL_RS05035 - hypothetical protein, at 679,295 to 679,510
_RS05035
SGL_RS05040: SGL_RS05040 - type II toxin-antitoxin system VapC family toxin, at 679,690 to 680,178
_RS05040
SGL_RS05050: SGL_RS05050 - lipid-A-disaccharide synthase-related protein, at 680,397 to 681,572
_RS05050
Position (kb) 679
680 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 678.540 kb on - strand, within SGL_RS05030 at 678.612 kb on - strand, within SGL_RS05030 at 678.680 kb on - strand, within SGL_RS05030 at 678.696 kb on - strand, within SGL_RS05030 at 678.768 kb on + strand, within SGL_RS05030 at 678.793 kb on - strand, within SGL_RS05030 at 678.793 kb on - strand, within SGL_RS05030 at 678.953 kb on + strand at 678.961 kb on - strand at 679.027 kb on - strand at 679.191 kb on + strand at 679.328 kb on + strand, within SGL_RS05035 at 679.573 kb on + strand at 679.647 kb on - strand at 679.807 kb on - strand, within SGL_RS05040 at 679.866 kb on + strand, within SGL_RS05040 at 679.874 kb on - strand, within SGL_RS05040 at 680.107 kb on + strand, within SGL_RS05040 at 680.130 kb on - strand at 680.358 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 678,540 - SGL_RS05030 0.35 +0.2 678,612 - SGL_RS05030 0.44 +0.4 678,680 - SGL_RS05030 0.54 -0.0 678,696 - SGL_RS05030 0.56 -1.1 678,768 + SGL_RS05030 0.65 -1.1 678,793 - SGL_RS05030 0.69 -1.3 678,793 - SGL_RS05030 0.69 +0.1 678,953 + +0.6 678,961 - +0.4 679,027 - +0.7 679,191 + +1.1 679,328 + SGL_RS05035 0.15 -3.6 679,573 + -1.7 679,647 - +0.6 679,807 - SGL_RS05040 0.24 +0.5 679,866 + SGL_RS05040 0.36 +0.1 679,874 - SGL_RS05040 0.38 +0.3 680,107 + SGL_RS05040 0.85 -0.4 680,130 - -0.9 680,358 + -1.1
Or see this region's nucleotide sequence