Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17300 and SGL_RS17305 are separated by 133 nucleotides SGL_RS17305 and SGL_RS17310 are separated by 14 nucleotides
SGL_RS17300: SGL_RS17300 - M3 family metallopeptidase, at 3,336,464 to 3,338,548
_RS17300
SGL_RS17305: SGL_RS17305 - pyrroline-5-carboxylate reductase, at 3,338,682 to 3,339,485
_RS17305
SGL_RS17310: SGL_RS17310 - glycoside hydrolase 100 family protein, at 3,339,500 to 3,340,969
_RS17310
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3338.027 kb on - strand, within SGL_RS17300 at 3338.071 kb on - strand, within SGL_RS17300 at 3338.162 kb on + strand, within SGL_RS17300 at 3338.294 kb on + strand, within SGL_RS17300 at 3338.354 kb on - strand at 3338.378 kb on + strand at 3338.536 kb on - strand at 3338.538 kb on + strand at 3338.546 kb on - strand at 3338.668 kb on - strand at 3338.668 kb on - strand at 3338.859 kb on - strand, within SGL_RS17305 at 3338.912 kb on - strand, within SGL_RS17305 at 3339.007 kb on - strand, within SGL_RS17305 at 3339.032 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.137 kb on + strand, within SGL_RS17305 at 3339.255 kb on + strand, within SGL_RS17305 at 3339.367 kb on + strand, within SGL_RS17305 at 3339.553 kb on - strand at 3339.819 kb on - strand, within SGL_RS17310 at 3339.922 kb on - strand, within SGL_RS17310 at 3339.950 kb on + strand, within SGL_RS17310 at 3340.169 kb on - strand, within SGL_RS17310 at 3340.280 kb on + strand, within SGL_RS17310 at 3340.313 kb on + strand, within SGL_RS17310 at 3340.448 kb on + strand, within SGL_RS17310
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 3,338,027 - SGL_RS17300 0.75 -0.8 3,338,071 - SGL_RS17300 0.77 +0.3 3,338,162 + SGL_RS17300 0.81 +0.3 3,338,294 + SGL_RS17300 0.88 +0.1 3,338,354 - +0.5 3,338,378 + -0.1 3,338,536 - -0.0 3,338,538 + -0.3 3,338,546 - -0.2 3,338,668 - -2.4 3,338,668 - -2.0 3,338,859 - SGL_RS17305 0.22 -1.2 3,338,912 - SGL_RS17305 0.29 -2.0 3,339,007 - SGL_RS17305 0.40 -1.1 3,339,032 + SGL_RS17305 0.44 -0.8 3,339,113 + SGL_RS17305 0.54 -4.1 3,339,113 + SGL_RS17305 0.54 -2.5 3,339,137 + SGL_RS17305 0.57 -0.9 3,339,255 + SGL_RS17305 0.71 -0.0 3,339,367 + SGL_RS17305 0.85 +0.4 3,339,553 - -0.4 3,339,819 - SGL_RS17310 0.22 +0.1 3,339,922 - SGL_RS17310 0.29 +0.2 3,339,950 + SGL_RS17310 0.31 +1.9 3,340,169 - SGL_RS17310 0.46 -0.4 3,340,280 + SGL_RS17310 0.53 -0.1 3,340,313 + SGL_RS17310 0.55 -0.3 3,340,448 + SGL_RS17310 0.64 -0.0
Or see this region's nucleotide sequence