Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11955 and SGL_RS11960 are separated by 105 nucleotides SGL_RS11960 and SGL_RS11965 overlap by 32 nucleotides SGL_RS11965 and SGL_RS11970 overlap by 8 nucleotides SGL_RS11970 and SGL_RS18940 are separated by 56 nucleotides SGL_RS18940 and SGL_RS11975 are separated by 101 nucleotides SGL_RS11975 and SGL_RS11980 are separated by 50 nucleotides
SGL_RS11955: SGL_RS11955 - aminomethyl-transferring glycine dehydrogenase, at 2,134,202 to 2,137,153
_RS11955
SGL_RS11960: SGL_RS11960 - hypothetical protein, at 2,137,259 to 2,137,738
_RS11960
SGL_RS11965: SGL_RS11965 - hypothetical protein, at 2,137,707 to 2,138,081
_RS11965
SGL_RS11970: SGL_RS11970 - HNH endonuclease, at 2,138,074 to 2,138,439
_RS11970
SGL_RS18940: SGL_RS18940 - DUF433 domain-containing protein, at 2,138,496 to 2,138,621
_RS18940
SGL_RS11975: SGL_RS11975 - DUF2358 domain-containing protein, at 2,138,723 to 2,139,142
_RS11975
SGL_RS11980: SGL_RS11980 - phosphoglycerate kinase, at 2,139,193 to 2,140,395
_RS11980
Position (kb)
2138
2139 Strain fitness (log2 ratio)
-1
0
1 at 2137.119 kb on + strand at 2137.292 kb on + strand at 2137.417 kb on + strand, within SGL_RS11960 at 2137.499 kb on - strand, within SGL_RS11960 at 2137.676 kb on + strand, within SGL_RS11960 at 2138.222 kb on + strand, within SGL_RS11970 at 2138.765 kb on + strand, within SGL_RS11975 at 2138.865 kb on - strand, within SGL_RS11975 at 2138.923 kb on + strand, within SGL_RS11975
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 2,137,119 + -0.1 2,137,292 + -0.1 2,137,417 + SGL_RS11960 0.33 +0.1 2,137,499 - SGL_RS11960 0.50 +1.0 2,137,676 + SGL_RS11960 0.87 +0.5 2,138,222 + SGL_RS11970 0.40 -1.0 2,138,765 + SGL_RS11975 0.10 +0.2 2,138,865 - SGL_RS11975 0.34 -0.1 2,138,923 + SGL_RS11975 0.48 -0.3
Or see this region's nucleotide sequence