Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11660 and SGL_RS11665 are separated by 90 nucleotides SGL_RS11665 and SGL_RS11670 overlap by 4 nucleotides SGL_RS11670 and SGL_RS11675 are separated by 166 nucleotides SGL_RS11675 and SGL_RS11680 overlap by 4 nucleotides SGL_RS11680 and SGL_RS11685 are separated by 39 nucleotides
SGL_RS11660: SGL_RS11660 - carotenoid oxygenase family protein, at 2,073,161 to 2,074,603
_RS11660
SGL_RS11665: SGL_RS11665 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 2,074,694 to 2,074,957
_RS11665
SGL_RS11670: SGL_RS11670 - Txe/YoeB family addiction module toxin, at 2,074,954 to 2,075,214
_RS11670
SGL_RS11675: SGL_RS11675 - chromophore lyase CpcT/CpeT, at 2,075,381 to 2,075,971
_RS11675
SGL_RS11680: SGL_RS11680 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 2,075,968 to 2,076,507
_RS11680
SGL_RS11685: SGL_RS11685 - diflavin flavoprotein, at 2,076,547 to 2,078,340
_RS11685
Position (kb)
2075
2076 Strain fitness (log2 ratio)
-1
0
1 at 2074.476 kb on - strand at 2075.585 kb on - strand, within SGL_RS11675 at 2075.592 kb on + strand, within SGL_RS11675 at 2075.791 kb on - strand, within SGL_RS11675 at 2075.832 kb on + strand, within SGL_RS11675 at 2076.057 kb on - strand, within SGL_RS11680 at 2076.632 kb on + strand at 2076.883 kb on + strand, within SGL_RS11685 at 2076.940 kb on + strand, within SGL_RS11685
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 2,074,476 - -0.0 2,075,585 - SGL_RS11675 0.35 -1.6 2,075,592 + SGL_RS11675 0.36 -0.7 2,075,791 - SGL_RS11675 0.69 -1.4 2,075,832 + SGL_RS11675 0.76 -1.4 2,076,057 - SGL_RS11680 0.16 -1.2 2,076,632 + -0.0 2,076,883 + SGL_RS11685 0.19 -0.5 2,076,940 + SGL_RS11685 0.22 +0.6
Or see this region's nucleotide sequence