Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09150 and SGL_RS09155 are separated by 308 nucleotides SGL_RS09155 and SGL_RS09160 are separated by 441 nucleotides SGL_RS09160 and SGL_RS09165 are separated by 110 nucleotides SGL_RS09165 and SGL_RS09170 are separated by 236 nucleotides
SGL_RS09150: SGL_RS09150 - acetolactate synthase large subunit, at 1,556,475 to 1,558,118
_RS09150
SGL_RS09155: SGL_RS09155 - riboflavin synthase subunit alpha, at 1,558,427 to 1,558,627
_RS09155
SGL_RS09160: SGL_RS09160 - thioredoxin family protein, at 1,559,069 to 1,559,611
_RS09160
SGL_RS09165: SGL_RS09165 - hypothetical protein, at 1,559,722 to 1,560,150
_RS09165
SGL_RS09170: SGL_RS09170 - class II fructose-bisphosphatase, at 1,560,387 to 1,561,424
_RS09170
Position (kb)
1559
1560 Strain fitness (log2 ratio)
-2
-1
0
1 at 1558.222 kb on + strand at 1558.266 kb on + strand at 1558.274 kb on - strand at 1558.669 kb on + strand at 1558.937 kb on - strand at 1559.104 kb on + strand at 1559.123 kb on - strand at 1559.170 kb on + strand, within SGL_RS09160 at 1559.393 kb on + strand, within SGL_RS09160 at 1559.776 kb on - strand, within SGL_RS09165 at 1559.907 kb on - strand, within SGL_RS09165 at 1559.947 kb on + strand, within SGL_RS09165 at 1559.955 kb on - strand, within SGL_RS09165 at 1560.019 kb on - strand, within SGL_RS09165 at 1560.050 kb on + strand, within SGL_RS09165 at 1560.058 kb on - strand, within SGL_RS09165 at 1560.123 kb on + strand at 1560.230 kb on - strand at 1560.292 kb on + strand at 1560.293 kb on - strand at 1560.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,558,222 + -0.0 1,558,266 + -0.1 1,558,274 - -0.1 1,558,669 + -0.8 1,558,937 - -0.1 1,559,104 + -0.2 1,559,123 - +0.4 1,559,170 + SGL_RS09160 0.19 -1.6 1,559,393 + SGL_RS09160 0.60 -1.9 1,559,776 - SGL_RS09165 0.13 -0.1 1,559,907 - SGL_RS09165 0.43 +0.1 1,559,947 + SGL_RS09165 0.52 -0.1 1,559,955 - SGL_RS09165 0.54 -0.1 1,560,019 - SGL_RS09165 0.69 +0.4 1,560,050 + SGL_RS09165 0.76 +0.3 1,560,058 - SGL_RS09165 0.78 +0.6 1,560,123 + +0.1 1,560,230 - -0.1 1,560,292 + -0.1 1,560,293 - -0.2 1,560,293 - -0.0
Or see this region's nucleotide sequence