Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04985 and SGL_RS04990 are separated by 56 nucleotides SGL_RS04990 and SGL_RS04995 are separated by 204 nucleotides SGL_RS04995 and SGL_RS05000 are separated by 46 nucleotides SGL_RS05000 and SGL_RS05005 are separated by 41 nucleotides
SGL_RS04985: SGL_RS04985 - reverse transcriptase-like protein, at 669,759 to 670,949
_RS04985
SGL_RS04990: SGL_RS04990 - class I SAM-dependent methyltransferase, at 671,006 to 671,707
_RS04990
SGL_RS04995: SGL_RS04995 - hypothetical protein, at 671,912 to 672,244
_RS04995
SGL_RS05000: SGL_RS05000 - signal peptidase I, at 672,291 to 672,947
_RS05000
SGL_RS05005: SGL_RS05005 - Uma2 family endonuclease, at 672,989 to 673,612
_RS05005
Position (kb) 671
672
673 Strain fitness (log2 ratio) -1
0
1 at 670.957 kb on + strand at 671.011 kb on + strand at 671.055 kb on - strand at 671.069 kb on + strand at 671.112 kb on - strand, within SGL_RS04990 at 671.141 kb on + strand, within SGL_RS04990 at 671.199 kb on - strand, within SGL_RS04990 at 671.232 kb on + strand, within SGL_RS04990 at 671.503 kb on - strand, within SGL_RS04990 at 671.586 kb on - strand, within SGL_RS04990 at 671.630 kb on - strand, within SGL_RS04990 at 671.841 kb on + strand at 671.855 kb on + strand at 671.855 kb on + strand at 671.863 kb on - strand at 671.863 kb on - strand at 671.896 kb on + strand at 671.896 kb on + strand at 671.978 kb on + strand, within SGL_RS04995 at 672.195 kb on - strand, within SGL_RS04995 at 672.848 kb on + strand, within SGL_RS05000
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 670,957 + -0.0 671,011 + +0.3 671,055 - +0.3 671,069 + +0.1 671,112 - SGL_RS04990 0.15 +0.2 671,141 + SGL_RS04990 0.19 -0.1 671,199 - SGL_RS04990 0.27 -0.2 671,232 + SGL_RS04990 0.32 +0.1 671,503 - SGL_RS04990 0.71 -0.1 671,586 - SGL_RS04990 0.83 -0.2 671,630 - SGL_RS04990 0.89 +0.3 671,841 + +0.4 671,855 + +0.2 671,855 + +0.1 671,863 - -0.3 671,863 - +0.1 671,896 + +0.4 671,896 + -0.3 671,978 + SGL_RS04995 0.20 -1.4 672,195 - SGL_RS04995 0.85 -0.4 672,848 + SGL_RS05000 0.85 +0.3
Or see this region's nucleotide sequence