Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01185 and SGL_RS01190 are separated by 91 nucleotides SGL_RS01190 and SGL_RS01195 are separated by 7 nucleotides SGL_RS01195 and SGL_RS01200 are separated by 21 nucleotides SGL_RS01200 and SGL_RS01205 are separated by 3 nucleotides
SGL_RS01185: SGL_RS01185 - hypothetical protein, at 9,205 to 9,621
_RS01185
SGL_RS01190: SGL_RS01190 - DUF2281 domain-containing protein, at 9,713 to 9,940
_RS01190
SGL_RS01195: SGL_RS01195 - PIN domain-containing protein, at 9,948 to 10,373
_RS01195
SGL_RS01200: SGL_RS01200 - ParA family protein, at 10,395 to 10,994
_RS01200
SGL_RS01205: SGL_RS01205 - ParB/RepB/Spo0J family partition protein, at 10,998 to 11,867
_RS01205
Position (kb)
10
11 Strain fitness (log2 ratio)
-2
-1
0
1 at 9.399 kb on - strand, within SGL_RS01185 at 9.399 kb on - strand, within SGL_RS01185 at 9.591 kb on - strand at 9.619 kb on - strand at 9.709 kb on + strand at 9.850 kb on + strand, within SGL_RS01190 at 9.873 kb on + strand, within SGL_RS01190 at 9.902 kb on + strand, within SGL_RS01190 at 10.046 kb on + strand, within SGL_RS01195 at 10.167 kb on - strand, within SGL_RS01195 at 10.255 kb on + strand, within SGL_RS01195 at 10.320 kb on + strand, within SGL_RS01195 at 10.320 kb on + strand, within SGL_RS01195 at 10.349 kb on + strand at 10.699 kb on + strand, within SGL_RS01200 at 10.744 kb on - strand, within SGL_RS01200 at 10.744 kb on - strand, within SGL_RS01200 at 10.801 kb on - strand, within SGL_RS01200 at 10.807 kb on - strand, within SGL_RS01200 at 10.839 kb on + strand, within SGL_RS01200 at 10.907 kb on + strand, within SGL_RS01200 at 10.907 kb on + strand, within SGL_RS01200 at 10.907 kb on + strand, within SGL_RS01200 at 10.915 kb on - strand, within SGL_RS01200 at 10.915 kb on - strand, within SGL_RS01200 at 10.915 kb on - strand, within SGL_RS01200 at 10.937 kb on - strand at 10.955 kb on + strand at 10.975 kb on + strand at 11.135 kb on + strand, within SGL_RS01205 at 11.156 kb on + strand, within SGL_RS01205 at 11.166 kb on + strand, within SGL_RS01205 at 11.222 kb on + strand, within SGL_RS01205 at 11.225 kb on - strand, within SGL_RS01205 at 11.263 kb on - strand, within SGL_RS01205 at 11.406 kb on + strand, within SGL_RS01205 at 11.457 kb on - strand, within SGL_RS01205 at 11.482 kb on - strand, within SGL_RS01205 at 11.731 kb on + strand, within SGL_RS01205 at 11.760 kb on + strand, within SGL_RS01205 at 11.772 kb on - strand, within SGL_RS01205 at 11.824 kb on - strand at 11.847 kb on - strand at 11.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 9,399 - SGL_RS01185 0.47 +0.6 9,399 - SGL_RS01185 0.47 -0.1 9,591 - -0.3 9,619 - +0.1 9,709 + -0.5 9,850 + SGL_RS01190 0.60 +0.2 9,873 + SGL_RS01190 0.70 -1.0 9,902 + SGL_RS01190 0.83 -0.3 10,046 + SGL_RS01195 0.23 +0.3 10,167 - SGL_RS01195 0.51 +0.1 10,255 + SGL_RS01195 0.72 +0.6 10,320 + SGL_RS01195 0.87 -0.9 10,320 + SGL_RS01195 0.87 +0.7 10,349 + +0.1 10,699 + SGL_RS01200 0.51 -1.5 10,744 - SGL_RS01200 0.58 -1.0 10,744 - SGL_RS01200 0.58 -0.3 10,801 - SGL_RS01200 0.68 -0.5 10,807 - SGL_RS01200 0.69 -0.5 10,839 + SGL_RS01200 0.74 -0.7 10,907 + SGL_RS01200 0.85 -1.1 10,907 + SGL_RS01200 0.85 -0.4 10,907 + SGL_RS01200 0.85 +0.2 10,915 - SGL_RS01200 0.87 +0.6 10,915 - SGL_RS01200 0.87 -0.5 10,915 - SGL_RS01200 0.87 -0.9 10,937 - -0.1 10,955 + -0.1 10,975 + -1.0 11,135 + SGL_RS01205 0.16 -0.6 11,156 + SGL_RS01205 0.18 -0.8 11,166 + SGL_RS01205 0.19 -0.7 11,222 + SGL_RS01205 0.26 -1.0 11,225 - SGL_RS01205 0.26 -0.4 11,263 - SGL_RS01205 0.30 -1.5 11,406 + SGL_RS01205 0.47 -1.6 11,457 - SGL_RS01205 0.53 +0.1 11,482 - SGL_RS01205 0.56 -1.1 11,731 + SGL_RS01205 0.84 -2.7 11,760 + SGL_RS01205 0.88 -0.1 11,772 - SGL_RS01205 0.89 -0.4 11,824 - -1.0 11,847 - +0.5 11,916 + +0.2
Or see this region's nucleotide sequence