Experiment: Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12970 and SGL_RS12975 are separated by 27 nucleotides SGL_RS12975 and SGL_RS12985 are separated by 255 nucleotides
SGL_RS12970: SGL_RS12970 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 2,385,351 to 2,385,911
_RS12970
SGL_RS12975: SGL_RS12975 - glycosyltransferase family 1 protein, at 2,385,939 to 2,387,168
_RS12975
SGL_RS12985: SGL_RS12985 - decarboxylating NADP(+)-dependent phosphogluconate dehydrogenase, at 2,387,424 to 2,388,851
_RS12985
Position (kb)
2385
2386
2387
2388 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2385.059 kb on + strand at 2385.099 kb on - strand at 2385.393 kb on + strand at 2385.566 kb on - strand, within SGL_RS12970 at 2385.607 kb on - strand, within SGL_RS12970 at 2385.716 kb on + strand, within SGL_RS12970 at 2385.724 kb on - strand, within SGL_RS12970 at 2385.729 kb on - strand, within SGL_RS12970 at 2385.799 kb on + strand, within SGL_RS12970 at 2385.821 kb on - strand, within SGL_RS12970 at 2385.839 kb on - strand, within SGL_RS12970 at 2385.913 kb on + strand at 2385.975 kb on + strand at 2385.975 kb on + strand at 2385.975 kb on + strand at 2385.977 kb on + strand at 2385.983 kb on - strand at 2385.983 kb on - strand at 2385.992 kb on + strand at 2386.024 kb on + strand at 2387.263 kb on - strand at 2387.272 kb on - strand at 2387.462 kb on + strand at 2387.462 kb on + strand at 2387.474 kb on + strand at 2387.869 kb on + strand, within SGL_RS12985 at 2387.903 kb on + strand, within SGL_RS12985 at 2387.919 kb on - strand, within SGL_RS12985 at 2387.969 kb on + strand, within SGL_RS12985
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 800 mM remove 2,385,059 + +0.1 2,385,099 - +0.0 2,385,393 + -0.0 2,385,566 - SGL_RS12970 0.38 -1.1 2,385,607 - SGL_RS12970 0.46 -1.2 2,385,716 + SGL_RS12970 0.65 -0.9 2,385,724 - SGL_RS12970 0.66 -0.2 2,385,729 - SGL_RS12970 0.67 -0.6 2,385,799 + SGL_RS12970 0.80 -1.3 2,385,821 - SGL_RS12970 0.84 -0.0 2,385,839 - SGL_RS12970 0.87 -0.4 2,385,913 + +0.0 2,385,975 + +0.5 2,385,975 + +0.1 2,385,975 + +0.1 2,385,977 + +0.3 2,385,983 - -0.8 2,385,983 - -0.9 2,385,992 + -0.0 2,386,024 + -1.4 2,387,263 - +0.4 2,387,272 - +0.3 2,387,462 + -2.1 2,387,462 + -2.4 2,387,474 + -0.5 2,387,869 + SGL_RS12985 0.31 -2.2 2,387,903 + SGL_RS12985 0.34 -4.0 2,387,919 - SGL_RS12985 0.35 -1.4 2,387,969 + SGL_RS12985 0.38 -3.0
Or see this region's nucleotide sequence