Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14655 and SGL_RS14660 are separated by 256 nucleotides SGL_RS14660 and SGL_RS19480 are separated by 94 nucleotides SGL_RS19480 and SGL_RS14665 are separated by 102 nucleotides
SGL_RS14655: SGL_RS14655 - efflux RND transporter periplasmic adaptor subunit, at 2,739,149 to 2,740,654
_RS14655
SGL_RS14660: SGL_RS14660 - 1,4-dihydroxy-2-naphthoyl-CoA hydrolase, at 2,740,911 to 2,741,327
_RS14660
SGL_RS19480: SGL_RS19480 - hypothetical protein, at 2,741,422 to 2,741,571
_RS19480
SGL_RS14665: SGL_RS14665 - glutamate--tRNA ligase, at 2,741,674 to 2,743,125
_RS14665
Position (kb)
2740
2741
2742 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2739.962 kb on + strand, within SGL_RS14655 at 2740.026 kb on + strand, within SGL_RS14655 at 2740.081 kb on - strand, within SGL_RS14655 at 2740.336 kb on - strand, within SGL_RS14655 at 2740.397 kb on - strand, within SGL_RS14655 at 2740.520 kb on - strand at 2740.551 kb on - strand at 2740.590 kb on + strand at 2740.590 kb on + strand at 2740.598 kb on - strand at 2740.620 kb on - strand at 2740.620 kb on - strand at 2740.863 kb on - strand at 2740.883 kb on - strand at 2740.997 kb on + strand, within SGL_RS14660 at 2741.073 kb on + strand, within SGL_RS14660 at 2741.079 kb on + strand, within SGL_RS14660 at 2741.159 kb on + strand, within SGL_RS14660 at 2741.321 kb on + strand at 2741.376 kb on - strand at 2741.402 kb on - strand at 2741.465 kb on - strand, within SGL_RS19480 at 2741.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,739,962 + SGL_RS14655 0.54 -0.5 2,740,026 + SGL_RS14655 0.58 -0.8 2,740,081 - SGL_RS14655 0.62 -1.5 2,740,336 - SGL_RS14655 0.79 -1.9 2,740,397 - SGL_RS14655 0.83 -1.3 2,740,520 - -0.0 2,740,551 - -2.1 2,740,590 + -0.9 2,740,590 + -0.8 2,740,598 - -0.2 2,740,620 - -0.4 2,740,620 - -0.6 2,740,863 - +1.2 2,740,883 - -0.6 2,740,997 + SGL_RS14660 0.21 +5.0 2,741,073 + SGL_RS14660 0.39 +5.7 2,741,079 + SGL_RS14660 0.40 +5.5 2,741,159 + SGL_RS14660 0.59 +6.4 2,741,321 + -1.9 2,741,376 - +0.9 2,741,402 - -1.5 2,741,465 - SGL_RS19480 0.29 -2.3 2,741,679 - +0.5
Or see this region's nucleotide sequence