Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14340 and SGL_RS14345 are separated by 130 nucleotides SGL_RS14345 and SGL_RS14350 are separated by 14 nucleotides SGL_RS14350 and SGL_RS14355 are separated by 10 nucleotides SGL_RS14355 and SGL_RS14360 are separated by 511 nucleotides
SGL_RS14340: SGL_RS14340 - protein phosphatase 2C domain-containing protein, at 2,671,895 to 2,673,970
_RS14340
SGL_RS14345: SGL_RS14345 - VWA domain-containing protein, at 2,674,101 to 2,674,721
_RS14345
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
SGL_RS14355: SGL_RS14355 - hypothetical protein, at 2,676,207 to 2,676,425
_RS14355
SGL_RS14360: SGL_RS14360 - carbon-nitrogen hydrolase family protein, at 2,676,937 to 2,677,755
_RS14360
Position (kb)
2674
2675
2676
2677 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2673.753 kb on - strand, within SGL_RS14340 at 2673.788 kb on + strand at 2673.881 kb on + strand at 2673.961 kb on - strand at 2673.967 kb on + strand at 2673.980 kb on - strand at 2673.980 kb on - strand at 2674.103 kb on - strand at 2674.215 kb on + strand, within SGL_RS14345 at 2674.334 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.376 kb on - strand, within SGL_RS14345 at 2674.724 kb on - strand at 2675.145 kb on + strand, within SGL_RS14350 at 2675.181 kb on - strand, within SGL_RS14350 at 2675.391 kb on + strand, within SGL_RS14350 at 2675.393 kb on + strand, within SGL_RS14350 at 2675.411 kb on - strand, within SGL_RS14350 at 2675.411 kb on - strand, within SGL_RS14350 at 2675.429 kb on + strand, within SGL_RS14350 at 2675.694 kb on - strand, within SGL_RS14350 at 2675.717 kb on - strand, within SGL_RS14350 at 2675.724 kb on - strand, within SGL_RS14350 at 2675.798 kb on - strand, within SGL_RS14350 at 2675.798 kb on - strand, within SGL_RS14350 at 2675.869 kb on - strand, within SGL_RS14350 at 2675.935 kb on - strand, within SGL_RS14350 at 2676.009 kb on + strand, within SGL_RS14350 at 2676.134 kb on - strand at 2676.153 kb on - strand at 2676.153 kb on - strand at 2676.213 kb on - strand at 2676.670 kb on - strand at 2676.894 kb on - strand at 2676.900 kb on + strand at 2676.900 kb on + strand at 2676.908 kb on - strand at 2676.910 kb on + strand at 2676.992 kb on - strand at 2677.055 kb on - strand, within SGL_RS14360 at 2677.088 kb on - strand, within SGL_RS14360 at 2677.183 kb on + strand, within SGL_RS14360
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,673,753 - SGL_RS14340 0.89 -0.7 2,673,788 + +0.7 2,673,881 + -0.2 2,673,961 - +4.1 2,673,967 + +2.6 2,673,980 - +4.9 2,673,980 - +5.0 2,674,103 - +1.8 2,674,215 + SGL_RS14345 0.18 +2.6 2,674,334 + SGL_RS14345 0.38 -1.6 2,674,368 + SGL_RS14345 0.43 -0.4 2,674,368 + SGL_RS14345 0.43 +0.3 2,674,376 - SGL_RS14345 0.44 +1.6 2,674,724 - -1.2 2,675,145 + SGL_RS14350 0.28 -2.0 2,675,181 - SGL_RS14350 0.30 +1.4 2,675,391 + SGL_RS14350 0.45 +0.3 2,675,393 + SGL_RS14350 0.45 -1.5 2,675,411 - SGL_RS14350 0.46 +2.4 2,675,411 - SGL_RS14350 0.46 -3.0 2,675,429 + SGL_RS14350 0.47 -1.2 2,675,694 - SGL_RS14350 0.66 +0.5 2,675,717 - SGL_RS14350 0.67 -1.8 2,675,724 - SGL_RS14350 0.68 -1.2 2,675,798 - SGL_RS14350 0.73 -3.2 2,675,798 - SGL_RS14350 0.73 -0.3 2,675,869 - SGL_RS14350 0.78 +3.2 2,675,935 - SGL_RS14350 0.82 -1.2 2,676,009 + SGL_RS14350 0.87 -1.4 2,676,134 - -2.7 2,676,153 - -1.9 2,676,153 - +0.1 2,676,213 - -2.0 2,676,670 - +0.0 2,676,894 - -0.2 2,676,900 + +3.0 2,676,900 + -0.9 2,676,908 - -1.2 2,676,910 + -0.4 2,676,992 - -2.1 2,677,055 - SGL_RS14360 0.14 -0.8 2,677,088 - SGL_RS14360 0.18 -0.6 2,677,183 + SGL_RS14360 0.30 -3.0
Or see this region's nucleotide sequence