Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12430 and SGL_RS12435 are separated by 38 nucleotides SGL_RS12435 and SGL_RS12440 are separated by 52 nucleotides SGL_RS12440 and SGL_RS12445 are separated by 235 nucleotides
SGL_RS12430: SGL_RS12430 - circadian clock protein KaiC, at 2,243,851 to 2,245,368
_RS12430
SGL_RS12435: SGL_RS12435 - PCP reductase family protein, at 2,245,407 to 2,245,583
_RS12435
SGL_RS12440: SGL_RS12440 - glycosyltransferase, at 2,245,636 to 2,246,631
_RS12440
SGL_RS12445: SGL_RS12445 - alpha/beta hydrolase, at 2,246,867 to 2,248,483
_RS12445
Position (kb)
2245
2246
2247 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2244.744 kb on + strand, within SGL_RS12430 at 2244.865 kb on + strand, within SGL_RS12430 at 2245.326 kb on - strand at 2245.533 kb on - strand, within SGL_RS12435 at 2245.647 kb on - strand at 2245.761 kb on + strand, within SGL_RS12440 at 2245.761 kb on + strand, within SGL_RS12440 at 2245.779 kb on - strand, within SGL_RS12440 at 2245.779 kb on - strand, within SGL_RS12440 at 2246.031 kb on - strand, within SGL_RS12440 at 2246.240 kb on + strand, within SGL_RS12440 at 2246.301 kb on + strand, within SGL_RS12440 at 2246.332 kb on + strand, within SGL_RS12440 at 2247.578 kb on - strand, within SGL_RS12445 at 2247.580 kb on - strand, within SGL_RS12445
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,244,744 + SGL_RS12430 0.59 -2.1 2,244,865 + SGL_RS12430 0.67 +3.5 2,245,326 - -2.7 2,245,533 - SGL_RS12435 0.71 -3.1 2,245,647 - -1.1 2,245,761 + SGL_RS12440 0.13 -4.1 2,245,761 + SGL_RS12440 0.13 -3.2 2,245,779 - SGL_RS12440 0.14 -1.6 2,245,779 - SGL_RS12440 0.14 -2.6 2,246,031 - SGL_RS12440 0.40 -1.1 2,246,240 + SGL_RS12440 0.61 -3.0 2,246,301 + SGL_RS12440 0.67 +1.1 2,246,332 + SGL_RS12440 0.70 -3.4 2,247,578 - SGL_RS12445 0.44 -0.6 2,247,580 - SGL_RS12445 0.44 -0.8
Or see this region's nucleotide sequence