Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10865 and SGL_RS10870 are separated by 100 nucleotides SGL_RS10870 and SGL_RS10875 are separated by 192 nucleotides
SGL_RS10865: SGL_RS10865 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB, at 1,902,437 to 1,903,924
_RS10865
SGL_RS10870: SGL_RS10870 - penicillin-binding protein 1A, at 1,904,025 to 1,905,977
_RS10870
SGL_RS10875: SGL_RS10875 - NAD(P)H-hydrate dehydratase, at 1,906,170 to 1,907,744
_RS10875
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1903.994 kb on + strand at 1904.002 kb on - strand at 1904.092 kb on + strand at 1904.285 kb on + strand, within SGL_RS10870 at 1904.293 kb on - strand, within SGL_RS10870 at 1904.293 kb on - strand, within SGL_RS10870 at 1904.425 kb on - strand, within SGL_RS10870 at 1904.450 kb on - strand, within SGL_RS10870 at 1904.481 kb on + strand, within SGL_RS10870 at 1904.483 kb on - strand, within SGL_RS10870 at 1904.488 kb on + strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.549 kb on - strand, within SGL_RS10870 at 1904.610 kb on - strand, within SGL_RS10870 at 1904.708 kb on - strand, within SGL_RS10870 at 1904.772 kb on + strand, within SGL_RS10870 at 1904.925 kb on + strand, within SGL_RS10870 at 1905.155 kb on - strand, within SGL_RS10870 at 1905.205 kb on - strand, within SGL_RS10870 at 1905.258 kb on + strand, within SGL_RS10870 at 1905.584 kb on - strand, within SGL_RS10870 at 1905.726 kb on - strand, within SGL_RS10870 at 1905.857 kb on + strand at 1905.858 kb on + strand at 1905.931 kb on + strand at 1905.939 kb on - strand at 1905.953 kb on + strand at 1905.987 kb on - strand at 1905.987 kb on - strand at 1906.082 kb on + strand at 1906.118 kb on + strand at 1906.126 kb on - strand at 1906.242 kb on + strand at 1906.504 kb on + strand, within SGL_RS10875 at 1906.733 kb on + strand, within SGL_RS10875 at 1906.736 kb on - strand, within SGL_RS10875 at 1906.748 kb on - strand, within SGL_RS10875 at 1906.766 kb on - strand, within SGL_RS10875 at 1906.800 kb on + strand, within SGL_RS10875 at 1906.858 kb on - strand, within SGL_RS10875 at 1906.870 kb on + strand, within SGL_RS10875 at 1906.870 kb on + strand, within SGL_RS10875 at 1906.933 kb on - strand, within SGL_RS10875
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,903,994 + -0.6 1,904,002 - -0.1 1,904,092 + -2.1 1,904,285 + SGL_RS10870 0.13 -1.6 1,904,293 - SGL_RS10870 0.14 -3.0 1,904,293 - SGL_RS10870 0.14 -3.4 1,904,425 - SGL_RS10870 0.20 -1.2 1,904,450 - SGL_RS10870 0.22 -2.3 1,904,481 + SGL_RS10870 0.23 -2.5 1,904,483 - SGL_RS10870 0.23 -1.7 1,904,488 + SGL_RS10870 0.24 -3.4 1,904,496 - SGL_RS10870 0.24 -2.8 1,904,496 - SGL_RS10870 0.24 -1.3 1,904,496 - SGL_RS10870 0.24 -2.9 1,904,549 - SGL_RS10870 0.27 -2.8 1,904,610 - SGL_RS10870 0.30 -4.4 1,904,708 - SGL_RS10870 0.35 -2.6 1,904,772 + SGL_RS10870 0.38 +2.2 1,904,925 + SGL_RS10870 0.46 -1.8 1,905,155 - SGL_RS10870 0.58 +0.5 1,905,205 - SGL_RS10870 0.60 -1.1 1,905,258 + SGL_RS10870 0.63 -2.1 1,905,584 - SGL_RS10870 0.80 -1.4 1,905,726 - SGL_RS10870 0.87 -2.0 1,905,857 + -2.5 1,905,858 + -1.8 1,905,931 + -0.7 1,905,939 - -4.1 1,905,953 + -3.2 1,905,987 - -1.5 1,905,987 - -0.4 1,906,082 + +0.3 1,906,118 + -0.3 1,906,126 - -0.5 1,906,242 + +0.7 1,906,504 + SGL_RS10875 0.21 -0.4 1,906,733 + SGL_RS10875 0.36 -1.0 1,906,736 - SGL_RS10875 0.36 -2.4 1,906,748 - SGL_RS10875 0.37 -0.6 1,906,766 - SGL_RS10875 0.38 -0.2 1,906,800 + SGL_RS10875 0.40 +0.8 1,906,858 - SGL_RS10875 0.44 -0.1 1,906,870 + SGL_RS10875 0.44 -0.7 1,906,870 + SGL_RS10875 0.44 -0.1 1,906,933 - SGL_RS10875 0.48 +0.9
Or see this region's nucleotide sequence