Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10225 and SGL_RS10230 are separated by 344 nucleotides SGL_RS10230 and SGL_RS10235 are separated by 142 nucleotides SGL_RS10235 and SGL_RS10240 are separated by 52 nucleotides
SGL_RS10225: SGL_RS10225 - EAL domain-containing protein, at 1,764,835 to 1,766,925
_RS10225
SGL_RS10230: SGL_RS10230 - orange carotenoid protein N-terminal domain-containing protein, at 1,767,270 to 1,768,223
_RS10230
SGL_RS10235: SGL_RS10235 - fluorescence recovery protein, at 1,768,366 to 1,768,695
_RS10235
SGL_RS10240: SGL_RS10240 - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 1,768,748 to 1,770,418
_RS10240
Position (kb)
1767
1768
1769 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1766.436 kb on + strand, within SGL_RS10225 at 1766.500 kb on + strand, within SGL_RS10225 at 1766.930 kb on - strand at 1767.288 kb on - strand at 1767.386 kb on + strand, within SGL_RS10230 at 1767.496 kb on + strand, within SGL_RS10230 at 1767.546 kb on + strand, within SGL_RS10230 at 1767.546 kb on + strand, within SGL_RS10230 at 1767.546 kb on + strand, within SGL_RS10230 at 1767.554 kb on - strand, within SGL_RS10230 at 1767.621 kb on + strand, within SGL_RS10230 at 1767.810 kb on + strand, within SGL_RS10230 at 1767.834 kb on - strand, within SGL_RS10230 at 1767.869 kb on - strand, within SGL_RS10230 at 1767.912 kb on - strand, within SGL_RS10230 at 1767.930 kb on - strand, within SGL_RS10230 at 1768.041 kb on - strand, within SGL_RS10230 at 1768.442 kb on - strand, within SGL_RS10235 at 1768.459 kb on + strand, within SGL_RS10235 at 1768.519 kb on + strand, within SGL_RS10235 at 1768.686 kb on - strand at 1768.722 kb on + strand at 1768.782 kb on + strand at 1768.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,766,436 + SGL_RS10225 0.77 -0.7 1,766,500 + SGL_RS10225 0.80 +2.2 1,766,930 - -0.3 1,767,288 - -0.3 1,767,386 + SGL_RS10230 0.12 -1.9 1,767,496 + SGL_RS10230 0.24 -1.3 1,767,546 + SGL_RS10230 0.29 +0.1 1,767,546 + SGL_RS10230 0.29 -1.4 1,767,546 + SGL_RS10230 0.29 -2.2 1,767,554 - SGL_RS10230 0.30 -0.9 1,767,621 + SGL_RS10230 0.37 -3.6 1,767,810 + SGL_RS10230 0.57 -3.7 1,767,834 - SGL_RS10230 0.59 -3.3 1,767,869 - SGL_RS10230 0.63 -0.8 1,767,912 - SGL_RS10230 0.67 -1.4 1,767,930 - SGL_RS10230 0.69 -3.2 1,768,041 - SGL_RS10230 0.81 -1.2 1,768,442 - SGL_RS10235 0.23 -1.2 1,768,459 + SGL_RS10235 0.28 -1.4 1,768,519 + SGL_RS10235 0.46 -2.0 1,768,686 - -2.6 1,768,722 + -1.7 1,768,782 + -1.2 1,768,889 + -0.5
Or see this region's nucleotide sequence