Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08085 and SGL_RS08090 are separated by 253 nucleotides SGL_RS08090 and SGL_RS08095 are separated by 118 nucleotides SGL_RS08095 and SGL_RS08100 are separated by 47 nucleotides SGL_RS08100 and SGL_RS08105 are separated by 151 nucleotides SGL_RS08105 and SGL_RS08110 are separated by 119 nucleotides
SGL_RS08085: SGL_RS08085 - DUF1499 domain-containing protein, at 1,326,150 to 1,326,626
_RS08085
SGL_RS08090: SGL_RS08090 - photosystem II reaction center protein Psb28, at 1,326,880 to 1,327,248
_RS08090
SGL_RS08095: SGL_RS08095 - peptide ABC transporter substrate-binding protein, at 1,327,367 to 1,329,115
_RS08095
SGL_RS08100: SGL_RS08100 - tRNA-Asp, at 1,329,163 to 1,329,236
_RS08100
SGL_RS08105: SGL_RS08105 - hypothetical protein, at 1,329,388 to 1,329,990
_RS08105
SGL_RS08110: SGL_RS08110 - type 1 glutamine amidotransferase, at 1,330,110 to 1,330,880
_RS08110
Position (kb)
1327
1328
1329
1330 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1326.455 kb on + strand, within SGL_RS08085 at 1326.575 kb on - strand, within SGL_RS08085 at 1326.652 kb on + strand at 1326.735 kb on + strand at 1326.791 kb on + strand at 1326.793 kb on + strand at 1326.835 kb on + strand at 1326.887 kb on + strand at 1326.946 kb on - strand, within SGL_RS08090 at 1326.979 kb on + strand, within SGL_RS08090 at 1326.987 kb on - strand, within SGL_RS08090 at 1327.017 kb on + strand, within SGL_RS08090 at 1327.395 kb on + strand at 1327.484 kb on + strand at 1327.789 kb on + strand, within SGL_RS08095 at 1327.797 kb on - strand, within SGL_RS08095 at 1327.882 kb on + strand, within SGL_RS08095 at 1328.197 kb on + strand, within SGL_RS08095 at 1328.214 kb on + strand, within SGL_RS08095 at 1328.257 kb on - strand, within SGL_RS08095 at 1328.398 kb on + strand, within SGL_RS08095 at 1328.424 kb on - strand, within SGL_RS08095 at 1328.684 kb on - strand, within SGL_RS08095 at 1328.779 kb on + strand, within SGL_RS08095 at 1329.012 kb on + strand at 1329.027 kb on + strand at 1329.131 kb on - strand at 1329.281 kb on + strand at 1329.361 kb on - strand at 1329.362 kb on - strand at 1329.471 kb on + strand, within SGL_RS08105 at 1329.471 kb on + strand, within SGL_RS08105 at 1329.471 kb on + strand, within SGL_RS08105 at 1329.544 kb on - strand, within SGL_RS08105 at 1329.556 kb on + strand, within SGL_RS08105 at 1329.610 kb on + strand, within SGL_RS08105 at 1329.694 kb on + strand, within SGL_RS08105 at 1329.832 kb on - strand, within SGL_RS08105 at 1329.864 kb on + strand, within SGL_RS08105 at 1329.871 kb on + strand, within SGL_RS08105 at 1329.937 kb on - strand at 1329.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,326,455 + SGL_RS08085 0.64 +0.7 1,326,575 - SGL_RS08085 0.89 -0.6 1,326,652 + +0.4 1,326,735 + +1.2 1,326,791 + -0.7 1,326,793 + +0.4 1,326,835 + -0.6 1,326,887 + -0.1 1,326,946 - SGL_RS08090 0.18 -0.5 1,326,979 + SGL_RS08090 0.27 -0.3 1,326,987 - SGL_RS08090 0.29 +0.3 1,327,017 + SGL_RS08090 0.37 -1.6 1,327,395 + -3.4 1,327,484 + -5.0 1,327,789 + SGL_RS08095 0.24 -3.8 1,327,797 - SGL_RS08095 0.25 -3.5 1,327,882 + SGL_RS08095 0.29 -1.8 1,328,197 + SGL_RS08095 0.47 -3.2 1,328,214 + SGL_RS08095 0.48 -4.3 1,328,257 - SGL_RS08095 0.51 -3.4 1,328,398 + SGL_RS08095 0.59 -3.6 1,328,424 - SGL_RS08095 0.60 -3.8 1,328,684 - SGL_RS08095 0.75 -5.2 1,328,779 + SGL_RS08095 0.81 -4.4 1,329,012 + -4.3 1,329,027 + -4.1 1,329,131 - -1.3 1,329,281 + -0.2 1,329,361 - -0.7 1,329,362 - -0.6 1,329,471 + SGL_RS08105 0.14 -2.3 1,329,471 + SGL_RS08105 0.14 -0.7 1,329,471 + SGL_RS08105 0.14 -2.7 1,329,544 - SGL_RS08105 0.26 -0.2 1,329,556 + SGL_RS08105 0.28 -0.6 1,329,610 + SGL_RS08105 0.37 -0.7 1,329,694 + SGL_RS08105 0.51 +1.7 1,329,832 - SGL_RS08105 0.74 -1.1 1,329,864 + SGL_RS08105 0.79 -1.4 1,329,871 + SGL_RS08105 0.80 -0.1 1,329,937 - -3.1 1,329,958 + -1.2
Or see this region's nucleotide sequence