Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07745 and SGL_RS07750 are separated by 84 nucleotides SGL_RS07750 and SGL_RS07755 are separated by 153 nucleotides SGL_RS07755 and SGL_RS07760 are separated by 180 nucleotides
SGL_RS07745: SGL_RS07745 - hypothetical protein, at 1,255,837 to 1,256,040
_RS07745
SGL_RS07750: SGL_RS07750 - 7-carboxy-7-deazaguanine synthase QueE, at 1,256,125 to 1,256,751
_RS07750
SGL_RS07755: SGL_RS07755 - UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase, at 1,256,905 to 1,258,263
_RS07755
SGL_RS07760: SGL_RS07760 - pitrilysin family protein, at 1,258,444 to 1,259,751
_RS07760
Position (kb) 1256
1257
1258
1259 Strain fitness (log2 ratio) -2
-1
0
1 at 1256.159 kb on + strand at 1256.367 kb on + strand, within SGL_RS07750 at 1256.379 kb on + strand, within SGL_RS07750 at 1256.387 kb on - strand, within SGL_RS07750 at 1256.459 kb on + strand, within SGL_RS07750 at 1256.470 kb on + strand, within SGL_RS07750 at 1256.480 kb on - strand, within SGL_RS07750 at 1256.480 kb on - strand, within SGL_RS07750 at 1256.487 kb on - strand, within SGL_RS07750 at 1256.501 kb on + strand, within SGL_RS07750 at 1256.583 kb on - strand, within SGL_RS07750 at 1256.588 kb on - strand, within SGL_RS07750 at 1256.606 kb on + strand, within SGL_RS07750 at 1256.677 kb on + strand, within SGL_RS07750 at 1256.685 kb on - strand, within SGL_RS07750 at 1256.716 kb on - strand at 1256.721 kb on - strand at 1256.801 kb on + strand at 1256.905 kb on - strand at 1258.615 kb on + strand, within SGL_RS07760 at 1259.054 kb on - strand, within SGL_RS07760 at 1259.103 kb on + strand, within SGL_RS07760
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,256,159 + +0.6 1,256,367 + SGL_RS07750 0.39 -1.0 1,256,379 + SGL_RS07750 0.41 +0.1 1,256,387 - SGL_RS07750 0.42 -1.5 1,256,459 + SGL_RS07750 0.53 -0.6 1,256,470 + SGL_RS07750 0.55 +0.9 1,256,480 - SGL_RS07750 0.57 -1.1 1,256,480 - SGL_RS07750 0.57 +0.8 1,256,487 - SGL_RS07750 0.58 -0.0 1,256,501 + SGL_RS07750 0.60 +0.4 1,256,583 - SGL_RS07750 0.73 -1.2 1,256,588 - SGL_RS07750 0.74 -2.6 1,256,606 + SGL_RS07750 0.77 -1.9 1,256,677 + SGL_RS07750 0.88 -1.6 1,256,685 - SGL_RS07750 0.89 -0.7 1,256,716 - -0.7 1,256,721 - -0.7 1,256,801 + -1.0 1,256,905 - -0.8 1,258,615 + SGL_RS07760 0.13 -0.2 1,259,054 - SGL_RS07760 0.47 -0.3 1,259,103 + SGL_RS07760 0.50 +0.9
Or see this region's nucleotide sequence