Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07540 and SGL_RS07545 are separated by 29 nucleotides SGL_RS07545 and SGL_RS07550 are separated by 54 nucleotides SGL_RS07550 and SGL_RS07555 are separated by 59 nucleotides SGL_RS07555 and SGL_RS07560 are separated by 137 nucleotides SGL_RS07560 and SGL_RS07565 are separated by 9 nucleotides
SGL_RS07540: SGL_RS07540 - endonuclease MutS2, at 1,217,156 to 1,219,624
_RS07540
SGL_RS07545: SGL_RS07545 - serine/threonine-protein phosphatase, at 1,219,654 to 1,220,418
_RS07545
SGL_RS07550: SGL_RS07550 - AarF/ABC1/UbiB kinase family protein, at 1,220,473 to 1,222,230
_RS07550
SGL_RS07555: SGL_RS07555 - hypothetical protein, at 1,222,290 to 1,222,472
_RS07555
SGL_RS07560: SGL_RS07560 - thylakoid lumen protein, at 1,222,610 to 1,222,927
_RS07560
SGL_RS07565: SGL_RS07565 - hypothetical protein, at 1,222,937 to 1,223,209
_RS07565
Position (kb)
1220
1221
1222
1223 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1219.632 kb on - strand at 1219.634 kb on - strand at 1219.660 kb on + strand at 1219.671 kb on + strand at 1219.765 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.841 kb on - strand, within SGL_RS07545 at 1219.928 kb on + strand, within SGL_RS07545 at 1219.934 kb on + strand, within SGL_RS07545 at 1219.942 kb on - strand, within SGL_RS07545 at 1219.957 kb on - strand, within SGL_RS07545 at 1220.109 kb on - strand, within SGL_RS07545 at 1220.260 kb on + strand, within SGL_RS07545 at 1220.322 kb on - strand, within SGL_RS07545 at 1220.465 kb on - strand at 1220.595 kb on - strand at 1220.659 kb on - strand, within SGL_RS07550 at 1220.690 kb on - strand, within SGL_RS07550 at 1220.695 kb on - strand, within SGL_RS07550 at 1220.732 kb on - strand, within SGL_RS07550 at 1220.763 kb on - strand, within SGL_RS07550 at 1220.828 kb on - strand, within SGL_RS07550 at 1221.002 kb on - strand, within SGL_RS07550 at 1221.089 kb on - strand, within SGL_RS07550 at 1221.108 kb on - strand, within SGL_RS07550 at 1221.198 kb on + strand, within SGL_RS07550 at 1221.206 kb on - strand, within SGL_RS07550 at 1221.301 kb on + strand, within SGL_RS07550 at 1221.717 kb on - strand, within SGL_RS07550 at 1221.722 kb on - strand, within SGL_RS07550 at 1221.727 kb on - strand, within SGL_RS07550 at 1221.778 kb on - strand, within SGL_RS07550 at 1221.804 kb on - strand, within SGL_RS07550 at 1221.926 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1222.415 kb on + strand, within SGL_RS07555 at 1222.613 kb on + strand at 1222.644 kb on + strand, within SGL_RS07560 at 1222.652 kb on - strand, within SGL_RS07560 at 1222.679 kb on - strand, within SGL_RS07560 at 1222.774 kb on - strand, within SGL_RS07560 at 1222.853 kb on + strand, within SGL_RS07560 at 1222.863 kb on - strand, within SGL_RS07560 at 1222.885 kb on + strand, within SGL_RS07560 at 1222.986 kb on + strand, within SGL_RS07565 at 1223.023 kb on - strand, within SGL_RS07565 at 1223.040 kb on + strand, within SGL_RS07565 at 1223.048 kb on - strand, within SGL_RS07565 at 1223.114 kb on + strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.132 kb on + strand, within SGL_RS07565 at 1223.158 kb on - strand, within SGL_RS07565
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,219,632 - +0.9 1,219,634 - +0.2 1,219,660 + +1.4 1,219,671 + +1.1 1,219,765 + SGL_RS07545 0.15 +2.1 1,219,833 + SGL_RS07545 0.23 -1.4 1,219,833 + SGL_RS07545 0.23 -1.2 1,219,841 - SGL_RS07545 0.24 -1.4 1,219,928 + SGL_RS07545 0.36 -3.4 1,219,934 + SGL_RS07545 0.37 -0.9 1,219,942 - SGL_RS07545 0.38 -1.6 1,219,957 - SGL_RS07545 0.40 -1.0 1,220,109 - SGL_RS07545 0.59 -2.0 1,220,260 + SGL_RS07545 0.79 -0.3 1,220,322 - SGL_RS07545 0.87 -0.3 1,220,465 - +0.7 1,220,595 - +1.3 1,220,659 - SGL_RS07550 0.11 +1.9 1,220,690 - SGL_RS07550 0.12 +1.4 1,220,695 - SGL_RS07550 0.13 +2.1 1,220,732 - SGL_RS07550 0.15 +1.3 1,220,763 - SGL_RS07550 0.16 +0.0 1,220,828 - SGL_RS07550 0.20 +2.0 1,221,002 - SGL_RS07550 0.30 +0.5 1,221,089 - SGL_RS07550 0.35 +1.4 1,221,108 - SGL_RS07550 0.36 +1.0 1,221,198 + SGL_RS07550 0.41 +1.8 1,221,206 - SGL_RS07550 0.42 +0.0 1,221,301 + SGL_RS07550 0.47 +0.3 1,221,717 - SGL_RS07550 0.71 +2.2 1,221,722 - SGL_RS07550 0.71 +1.3 1,221,727 - SGL_RS07550 0.71 +1.4 1,221,778 - SGL_RS07550 0.74 +1.3 1,221,804 - SGL_RS07550 0.76 +1.8 1,221,926 + SGL_RS07550 0.83 +2.2 1,221,942 + SGL_RS07550 0.84 +1.8 1,221,942 + SGL_RS07550 0.84 +1.2 1,222,415 + SGL_RS07555 0.68 +1.0 1,222,613 + -0.3 1,222,644 + SGL_RS07560 0.11 +0.7 1,222,652 - SGL_RS07560 0.13 -0.4 1,222,679 - SGL_RS07560 0.22 +0.2 1,222,774 - SGL_RS07560 0.52 -1.5 1,222,853 + SGL_RS07560 0.76 -1.8 1,222,863 - SGL_RS07560 0.80 -3.9 1,222,885 + SGL_RS07560 0.86 -1.5 1,222,986 + SGL_RS07565 0.18 -1.4 1,223,023 - SGL_RS07565 0.32 -3.6 1,223,040 + SGL_RS07565 0.38 -4.2 1,223,048 - SGL_RS07565 0.41 -4.6 1,223,114 + SGL_RS07565 0.65 -2.1 1,223,122 - SGL_RS07565 0.68 -3.4 1,223,122 - SGL_RS07565 0.68 -1.8 1,223,132 + SGL_RS07565 0.71 -1.9 1,223,158 - SGL_RS07565 0.81 -4.1
Or see this region's nucleotide sequence