Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07250 and SGL_RS07260 are separated by 228 nucleotides SGL_RS07260 and SGL_RS07265 are separated by 20 nucleotides SGL_RS07265 and SGL_RS07270 are separated by 87 nucleotides SGL_RS07270 and SGL_RS07275 are separated by 76 nucleotides SGL_RS07275 and SGL_RS07280 are separated by 53 nucleotides
SGL_RS07250: SGL_RS07250 - aminotransferase class IV, at 1,153,292 to 1,154,101
_RS07250
SGL_RS07260: SGL_RS07260 - hypothetical protein, at 1,154,330 to 1,154,836
_RS07260
SGL_RS07265: SGL_RS07265 - AarF/ABC1/UbiB kinase family protein, at 1,154,857 to 1,156,083
_RS07265
SGL_RS07270: SGL_RS07270 - hypothetical protein, at 1,156,171 to 1,156,503
_RS07270
SGL_RS07275: SGL_RS07275 - clan AA aspartic protease, at 1,156,580 to 1,156,975
_RS07275
SGL_RS07280: SGL_RS07280 - excinuclease ABC subunit UvrC, at 1,157,029 to 1,158,909
_RS07280
Position (kb) 1154
1155
1156
1157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1153.953 kb on - strand, within SGL_RS07250 at 1154.061 kb on - strand at 1154.306 kb on - strand at 1154.663 kb on - strand, within SGL_RS07260 at 1154.689 kb on - strand, within SGL_RS07260 at 1154.689 kb on - strand, within SGL_RS07260 at 1154.696 kb on + strand, within SGL_RS07260 at 1154.712 kb on + strand, within SGL_RS07260 at 1154.741 kb on + strand, within SGL_RS07260 at 1154.763 kb on - strand, within SGL_RS07260 at 1154.797 kb on - strand at 1154.803 kb on - strand at 1154.806 kb on - strand at 1154.820 kb on - strand at 1154.869 kb on + strand at 1154.898 kb on + strand at 1154.949 kb on - strand at 1155.126 kb on - strand, within SGL_RS07265 at 1155.136 kb on + strand, within SGL_RS07265 at 1155.190 kb on - strand, within SGL_RS07265 at 1155.595 kb on - strand, within SGL_RS07265 at 1155.779 kb on + strand, within SGL_RS07265 at 1156.304 kb on - strand, within SGL_RS07270
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,153,953 - SGL_RS07250 0.82 +0.5 1,154,061 - +2.5 1,154,306 - +0.1 1,154,663 - SGL_RS07260 0.66 +0.3 1,154,689 - SGL_RS07260 0.71 +0.3 1,154,689 - SGL_RS07260 0.71 -0.9 1,154,696 + SGL_RS07260 0.72 -2.3 1,154,712 + SGL_RS07260 0.75 -0.8 1,154,741 + SGL_RS07260 0.81 +0.3 1,154,763 - SGL_RS07260 0.85 +0.5 1,154,797 - -0.2 1,154,803 - -1.3 1,154,806 - -3.6 1,154,820 - -0.2 1,154,869 + -2.6 1,154,898 + -1.6 1,154,949 - +0.3 1,155,126 - SGL_RS07265 0.22 +1.2 1,155,136 + SGL_RS07265 0.23 -0.4 1,155,190 - SGL_RS07265 0.27 +1.0 1,155,595 - SGL_RS07265 0.60 -1.6 1,155,779 + SGL_RS07265 0.75 +0.2 1,156,304 - SGL_RS07270 0.40 -0.1
Or see this region's nucleotide sequence