Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06720 and SGL_RS06725 are separated by 359 nucleotides SGL_RS06725 and SGL_RS06730 are separated by 175 nucleotides SGL_RS06730 and SGL_RS06735 are separated by 131 nucleotides SGL_RS06735 and SGL_RS06740 are separated by 51 nucleotides SGL_RS06740 and SGL_RS06745 are separated by 45 nucleotides
SGL_RS06720: SGL_RS06720 - SH3 domain-containing protein, at 1,039,196 to 1,039,732
_RS06720
SGL_RS06725: SGL_RS06725 - hypothetical protein, at 1,040,092 to 1,040,355
_RS06725
SGL_RS06730: SGL_RS06730 - succinate dehydrogenase/fumarate reductase flavoprotein subunit, at 1,040,531 to 1,042,258
_RS06730
SGL_RS06735: SGL_RS06735 - PCP reductase family protein, at 1,042,390 to 1,042,557
_RS06735
SGL_RS06740: SGL_RS06740 - histidine triad nucleotide-binding protein, at 1,042,609 to 1,042,953
_RS06740
SGL_RS06745: SGL_RS06745 - alpha/beta fold hydrolase, at 1,042,999 to 1,043,880
_RS06745
Position (kb)
1040
1041
1042
1043 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1039.632 kb on - strand, within SGL_RS06720 at 1039.644 kb on + strand, within SGL_RS06720 at 1039.691 kb on - strand at 1039.691 kb on - strand at 1039.692 kb on + strand at 1039.858 kb on + strand at 1039.891 kb on + strand at 1039.899 kb on - strand at 1040.147 kb on - strand, within SGL_RS06725 at 1040.185 kb on + strand, within SGL_RS06725 at 1040.218 kb on - strand, within SGL_RS06725 at 1040.248 kb on - strand, within SGL_RS06725 at 1040.473 kb on + strand at 1040.613 kb on + strand at 1040.632 kb on + strand at 1040.734 kb on - strand, within SGL_RS06730 at 1040.755 kb on + strand, within SGL_RS06730 at 1041.175 kb on - strand, within SGL_RS06730 at 1041.327 kb on + strand, within SGL_RS06730 at 1041.559 kb on + strand, within SGL_RS06730 at 1041.564 kb on + strand, within SGL_RS06730 at 1041.736 kb on - strand, within SGL_RS06730 at 1041.746 kb on + strand, within SGL_RS06730 at 1041.746 kb on + strand, within SGL_RS06730 at 1041.754 kb on - strand, within SGL_RS06730 at 1041.927 kb on - strand, within SGL_RS06730 at 1041.982 kb on - strand, within SGL_RS06730 at 1042.046 kb on - strand, within SGL_RS06730 at 1042.061 kb on - strand, within SGL_RS06730 at 1042.170 kb on + strand at 1042.668 kb on - strand, within SGL_RS06740 at 1042.690 kb on + strand, within SGL_RS06740 at 1042.806 kb on + strand, within SGL_RS06740 at 1042.839 kb on - strand, within SGL_RS06740 at 1042.846 kb on - strand, within SGL_RS06740 at 1042.955 kb on + strand at 1043.126 kb on + strand, within SGL_RS06745 at 1043.170 kb on + strand, within SGL_RS06745 at 1043.190 kb on - strand, within SGL_RS06745 at 1043.200 kb on + strand, within SGL_RS06745 at 1043.217 kb on - strand, within SGL_RS06745 at 1043.244 kb on - strand, within SGL_RS06745
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,039,632 - SGL_RS06720 0.81 +1.6 1,039,644 + SGL_RS06720 0.83 -0.4 1,039,691 - -0.5 1,039,691 - -0.4 1,039,692 + +0.2 1,039,858 + +1.0 1,039,891 + -0.8 1,039,899 - -0.2 1,040,147 - SGL_RS06725 0.21 -4.2 1,040,185 + SGL_RS06725 0.35 -3.9 1,040,218 - SGL_RS06725 0.48 -2.7 1,040,248 - SGL_RS06725 0.59 -4.0 1,040,473 + -0.4 1,040,613 + -1.0 1,040,632 + -0.4 1,040,734 - SGL_RS06730 0.12 +0.6 1,040,755 + SGL_RS06730 0.13 -0.6 1,041,175 - SGL_RS06730 0.37 -1.9 1,041,327 + SGL_RS06730 0.46 -1.2 1,041,559 + SGL_RS06730 0.59 -0.2 1,041,564 + SGL_RS06730 0.60 -2.3 1,041,736 - SGL_RS06730 0.70 +0.1 1,041,746 + SGL_RS06730 0.70 -2.4 1,041,746 + SGL_RS06730 0.70 -1.0 1,041,754 - SGL_RS06730 0.71 +2.0 1,041,927 - SGL_RS06730 0.81 -1.6 1,041,982 - SGL_RS06730 0.84 -0.8 1,042,046 - SGL_RS06730 0.88 -1.3 1,042,061 - SGL_RS06730 0.89 -0.5 1,042,170 + -0.3 1,042,668 - SGL_RS06740 0.17 -1.0 1,042,690 + SGL_RS06740 0.23 +1.8 1,042,806 + SGL_RS06740 0.57 -1.6 1,042,839 - SGL_RS06740 0.67 -1.2 1,042,846 - SGL_RS06740 0.69 +0.4 1,042,955 + +0.5 1,043,126 + SGL_RS06745 0.14 -0.1 1,043,170 + SGL_RS06745 0.19 +0.6 1,043,190 - SGL_RS06745 0.22 +0.7 1,043,200 + SGL_RS06745 0.23 +0.7 1,043,217 - SGL_RS06745 0.25 +0.4 1,043,244 - SGL_RS06745 0.28 +0.2
Or see this region's nucleotide sequence