Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06405 and SGL_RS06410 are separated by 61 nucleotides SGL_RS06410 and SGL_RS06415 are separated by 275 nucleotides SGL_RS06415 and SGL_RS06420 are separated by 200 nucleotides
SGL_RS06405: SGL_RS06405 - threonine-phosphate decarboxylase CobD, at 969,762 to 970,820
_RS06405
SGL_RS06410: SGL_RS06410 - HU family DNA-binding protein, at 970,882 to 971,184
_RS06410
SGL_RS06415: SGL_RS06415 - GNAT family N-acetyltransferase, at 971,460 to 972,284
_RS06415
SGL_RS06420: SGL_RS06420 - hypothetical protein, at 972,485 to 973,954
_RS06420
Position (kb) 971
972
973 Strain fitness (log2 ratio) -2
-1
0
1
2 at 971.475 kb on + strand at 971.732 kb on + strand, within SGL_RS06415 at 971.734 kb on + strand, within SGL_RS06415 at 972.020 kb on + strand, within SGL_RS06415 at 972.028 kb on - strand, within SGL_RS06415 at 972.028 kb on - strand, within SGL_RS06415 at 972.170 kb on - strand, within SGL_RS06415 at 972.229 kb on + strand at 972.286 kb on + strand at 972.325 kb on - strand at 972.461 kb on + strand at 972.517 kb on - strand at 972.590 kb on - strand at 972.623 kb on + strand at 972.631 kb on - strand at 972.631 kb on - strand at 972.668 kb on + strand, within SGL_RS06420 at 972.695 kb on + strand, within SGL_RS06420 at 972.804 kb on + strand, within SGL_RS06420 at 972.945 kb on - strand, within SGL_RS06420 at 973.238 kb on - strand, within SGL_RS06420
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 971,475 + -1.1 971,732 + SGL_RS06415 0.33 +2.3 971,734 + SGL_RS06415 0.33 +0.9 972,020 + SGL_RS06415 0.68 +1.6 972,028 - SGL_RS06415 0.69 +1.3 972,028 - SGL_RS06415 0.69 +0.9 972,170 - SGL_RS06415 0.86 +1.1 972,229 + -0.3 972,286 + +0.8 972,325 - +0.4 972,461 + -0.9 972,517 - -0.9 972,590 - -0.6 972,623 + -1.3 972,631 - +0.2 972,631 - +1.8 972,668 + SGL_RS06420 0.12 -0.4 972,695 + SGL_RS06420 0.14 -2.6 972,804 + SGL_RS06420 0.22 +0.8 972,945 - SGL_RS06420 0.31 +0.2 973,238 - SGL_RS06420 0.51 -0.5
Or see this region's nucleotide sequence