Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05285 and SGL_RS05290 are separated by 116 nucleotides SGL_RS05290 and SGL_RS05295 are separated by 232 nucleotides SGL_RS05295 and SGL_RS05300 are separated by 125 nucleotides
SGL_RS05285: SGL_RS05285 - aspartoacylase, at 732,418 to 733,284
_RS05285
SGL_RS05290: SGL_RS05290 - NAD-dependent epimerase/dehydratase family protein, at 733,401 to 734,432
_RS05290
SGL_RS05295: SGL_RS05295 - heavy-metal-associated domain-containing protein, at 734,665 to 734,859
_RS05295
SGL_RS05300: SGL_RS05300 - M23 family metallopeptidase, at 734,985 to 736,220
_RS05300
Position (kb) 733
734
735 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 732.617 kb on + strand, within SGL_RS05285 at 732.781 kb on - strand, within SGL_RS05285 at 732.802 kb on + strand, within SGL_RS05285 at 732.827 kb on - strand, within SGL_RS05285 at 732.892 kb on + strand, within SGL_RS05285 at 732.983 kb on + strand, within SGL_RS05285 at 733.205 kb on - strand at 733.253 kb on + strand at 733.393 kb on - strand at 733.458 kb on + strand at 733.473 kb on + strand at 733.507 kb on - strand, within SGL_RS05290 at 733.631 kb on + strand, within SGL_RS05290 at 733.677 kb on + strand, within SGL_RS05290 at 733.846 kb on + strand, within SGL_RS05290 at 733.872 kb on + strand, within SGL_RS05290 at 734.023 kb on - strand, within SGL_RS05290 at 734.207 kb on + strand, within SGL_RS05290 at 734.721 kb on + strand, within SGL_RS05295 at 734.766 kb on - strand, within SGL_RS05295 at 734.800 kb on + strand, within SGL_RS05295 at 734.801 kb on - strand, within SGL_RS05295 at 734.869 kb on - strand at 734.869 kb on - strand at 734.869 kb on - strand at 734.956 kb on + strand at 734.958 kb on + strand at 735.034 kb on + strand at 735.144 kb on + strand, within SGL_RS05300 at 735.334 kb on + strand, within SGL_RS05300
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 732,617 + SGL_RS05285 0.23 -0.7 732,781 - SGL_RS05285 0.42 -2.7 732,802 + SGL_RS05285 0.44 -0.4 732,827 - SGL_RS05285 0.47 -1.6 732,892 + SGL_RS05285 0.55 -2.8 732,983 + SGL_RS05285 0.65 -2.0 733,205 - -0.7 733,253 + -0.5 733,393 - +0.3 733,458 + -0.8 733,473 + -4.1 733,507 - SGL_RS05290 0.10 -3.0 733,631 + SGL_RS05290 0.22 -1.5 733,677 + SGL_RS05290 0.27 -4.2 733,846 + SGL_RS05290 0.43 -3.8 733,872 + SGL_RS05290 0.46 -2.3 734,023 - SGL_RS05290 0.60 -2.4 734,207 + SGL_RS05290 0.78 -3.1 734,721 + SGL_RS05295 0.29 -1.2 734,766 - SGL_RS05295 0.52 +0.2 734,800 + SGL_RS05295 0.69 +0.2 734,801 - SGL_RS05295 0.70 -1.5 734,869 - -1.0 734,869 - +0.3 734,869 - +0.1 734,956 + -1.2 734,958 + -1.7 735,034 + -3.3 735,144 + SGL_RS05300 0.13 +1.3 735,334 + SGL_RS05300 0.28 -1.1
Or see this region's nucleotide sequence