Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04780 and SGL_RS04785 are separated by 149 nucleotides SGL_RS04785 and SGL_RS04790 are separated by 52 nucleotides SGL_RS04790 and SGL_RS04795 are separated by 12 nucleotides SGL_RS04795 and SGL_RS04800 are separated by 304 nucleotides
SGL_RS04780: SGL_RS04780 - alpha/beta fold hydrolase, at 620,560 to 621,468
_RS04780
SGL_RS04785: SGL_RS04785 - CPP1-like family protein, at 621,618 to 622,247
_RS04785
SGL_RS04790: SGL_RS04790 - penicillin-binding protein 2, at 622,300 to 624,123
_RS04790
SGL_RS04795: SGL_RS04795 - hypothetical protein, at 624,136 to 624,594
_RS04795
SGL_RS04800: SGL_RS04800 - (Fe-S)-binding protein, at 624,899 to 626,281
_RS04800
Position (kb) 622
623
624
625 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 621.354 kb on + strand, within SGL_RS04780 at 621.362 kb on + strand, within SGL_RS04780 at 621.411 kb on - strand at 621.449 kb on + strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.462 kb on + strand at 621.462 kb on + strand at 621.481 kb on - strand at 621.483 kb on - strand at 621.531 kb on + strand at 621.531 kb on - strand at 621.608 kb on + strand at 621.711 kb on + strand, within SGL_RS04785 at 621.916 kb on - strand, within SGL_RS04785 at 622.013 kb on - strand, within SGL_RS04785 at 622.174 kb on + strand, within SGL_RS04785 at 622.308 kb on - strand at 622.317 kb on + strand at 624.257 kb on + strand, within SGL_RS04795 at 625.107 kb on + strand, within SGL_RS04800
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 621,354 + SGL_RS04780 0.87 -0.2 621,362 + SGL_RS04780 0.88 -2.6 621,411 - -1.7 621,449 + -0.2 621,457 - +0.4 621,457 - +0.3 621,457 - +1.1 621,462 + +0.4 621,462 + -1.1 621,481 - -2.6 621,483 - -0.7 621,531 + +1.1 621,531 - -0.6 621,608 + -1.3 621,711 + SGL_RS04785 0.15 -2.0 621,916 - SGL_RS04785 0.47 -2.5 622,013 - SGL_RS04785 0.63 +0.3 622,174 + SGL_RS04785 0.88 -2.3 622,308 - +0.0 622,317 + -4.1 624,257 + SGL_RS04795 0.26 +1.3 625,107 + SGL_RS04800 0.15 -3.4
Or see this region's nucleotide sequence