Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03635 and SGL_RS03640 overlap by 4 nucleotides SGL_RS03640 and SGL_RS03645 are separated by 301 nucleotides SGL_RS03645 and SGL_RS03650 are separated by 63 nucleotides
SGL_RS03635: SGL_RS03635 - glycosyltransferase family 4 protein, at 370,285 to 371,490
_RS03635
SGL_RS03640: SGL_RS03640 - UDP-glucose 4-epimerase GalE, at 371,487 to 372,509
_RS03640
SGL_RS03645: SGL_RS03645 - FkbM family methyltransferase, at 372,811 to 373,167
_RS03645
SGL_RS03650: SGL_RS03650 - FkbM family methyltransferase, at 373,231 to 373,644
_RS03650
Position (kb) 371
372
373 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 370.627 kb on - strand, within SGL_RS03635 at 370.984 kb on - strand, within SGL_RS03635 at 371.381 kb on + strand at 371.389 kb on - strand at 371.389 kb on - strand at 371.393 kb on + strand at 371.503 kb on - strand at 371.571 kb on + strand at 371.582 kb on - strand at 371.642 kb on - strand, within SGL_RS03640 at 372.012 kb on - strand, within SGL_RS03640 at 372.196 kb on - strand, within SGL_RS03640 at 372.366 kb on + strand, within SGL_RS03640 at 372.366 kb on + strand, within SGL_RS03640 at 372.376 kb on + strand, within SGL_RS03640 at 372.612 kb on + strand at 373.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 370,627 - SGL_RS03635 0.28 -5.6 370,984 - SGL_RS03635 0.58 -1.8 371,381 + -6.6 371,389 - -4.4 371,389 - -4.5 371,393 + -2.8 371,503 - -4.3 371,571 + -3.4 371,582 - -2.9 371,642 - SGL_RS03640 0.15 -3.7 372,012 - SGL_RS03640 0.51 -4.8 372,196 - SGL_RS03640 0.69 -4.2 372,366 + SGL_RS03640 0.86 -4.2 372,366 + SGL_RS03640 0.86 -3.0 372,376 + SGL_RS03640 0.87 -4.2 372,612 + -1.5 373,147 - +1.5
Or see this region's nucleotide sequence