Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03270 and SGL_RS20110 are separated by 245 nucleotides SGL_RS20110 and SGL_RS03275 are separated by 564 nucleotides SGL_RS03275 and SGL_RS03280 are separated by 226 nucleotides
SGL_RS03270: SGL_RS03270 - HNH endonuclease, at 298,083 to 298,580
_RS03270
SGL_RS20110: SGL_RS20110 - hypothetical protein, at 298,826 to 298,957
_RS20110
SGL_RS03275: SGL_RS03275 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 299,522 to 300,883
_RS03275
SGL_RS03280: SGL_RS03280 - FAD-dependent hydroxylase, at 301,110 to 302,354
_RS03280
Position (kb) 299
300
301 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 298.536 kb on + strand at 298.870 kb on + strand, within SGL_RS20110 at 298.946 kb on + strand at 299.106 kb on - strand at 299.270 kb on + strand at 299.312 kb on - strand at 299.391 kb on - strand at 299.546 kb on + strand at 299.645 kb on + strand at 299.653 kb on - strand at 299.659 kb on + strand, within SGL_RS03275 at 299.739 kb on - strand, within SGL_RS03275 at 299.741 kb on + strand, within SGL_RS03275 at 299.749 kb on - strand, within SGL_RS03275 at 299.833 kb on + strand, within SGL_RS03275 at 299.841 kb on - strand, within SGL_RS03275 at 299.843 kb on - strand, within SGL_RS03275 at 299.843 kb on - strand, within SGL_RS03275 at 299.868 kb on - strand, within SGL_RS03275 at 299.890 kb on + strand, within SGL_RS03275 at 299.960 kb on + strand, within SGL_RS03275 at 299.973 kb on - strand, within SGL_RS03275 at 300.026 kb on + strand, within SGL_RS03275 at 300.051 kb on + strand, within SGL_RS03275 at 300.074 kb on + strand, within SGL_RS03275 at 300.080 kb on + strand, within SGL_RS03275 at 300.199 kb on + strand, within SGL_RS03275 at 300.281 kb on - strand, within SGL_RS03275 at 300.399 kb on - strand, within SGL_RS03275 at 300.407 kb on + strand, within SGL_RS03275 at 300.414 kb on - strand, within SGL_RS03275 at 300.581 kb on - strand, within SGL_RS03275 at 300.583 kb on + strand, within SGL_RS03275 at 300.710 kb on + strand, within SGL_RS03275 at 300.781 kb on - strand at 300.783 kb on + strand at 300.791 kb on - strand at 300.869 kb on - strand at 300.893 kb on - strand at 300.941 kb on - strand at 300.963 kb on - strand at 301.045 kb on + strand at 301.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 298,536 + +2.5 298,870 + SGL_RS20110 0.33 +1.3 298,946 + +0.6 299,106 - +0.4 299,270 + -0.4 299,312 - +0.3 299,391 - -0.4 299,546 + +0.2 299,645 + -3.0 299,653 - -2.4 299,659 + SGL_RS03275 0.10 -3.0 299,739 - SGL_RS03275 0.16 -1.9 299,741 + SGL_RS03275 0.16 -3.7 299,749 - SGL_RS03275 0.17 -2.1 299,833 + SGL_RS03275 0.23 -3.7 299,841 - SGL_RS03275 0.23 -2.9 299,843 - SGL_RS03275 0.24 -5.1 299,843 - SGL_RS03275 0.24 -1.8 299,868 - SGL_RS03275 0.25 -0.4 299,890 + SGL_RS03275 0.27 +0.9 299,960 + SGL_RS03275 0.32 -1.9 299,973 - SGL_RS03275 0.33 -1.6 300,026 + SGL_RS03275 0.37 +1.2 300,051 + SGL_RS03275 0.39 -4.5 300,074 + SGL_RS03275 0.41 -4.3 300,080 + SGL_RS03275 0.41 -1.0 300,199 + SGL_RS03275 0.50 -2.1 300,281 - SGL_RS03275 0.56 -0.9 300,399 - SGL_RS03275 0.64 -2.8 300,407 + SGL_RS03275 0.65 -2.6 300,414 - SGL_RS03275 0.65 -2.9 300,581 - SGL_RS03275 0.78 -0.4 300,583 + SGL_RS03275 0.78 +0.2 300,710 + SGL_RS03275 0.87 -0.8 300,781 - -3.7 300,783 + -3.4 300,791 - -2.6 300,869 - -1.8 300,893 - -0.0 300,941 - -0.2 300,963 - +0.2 301,045 + +7.6 301,115 - +0.9
Or see this region's nucleotide sequence