Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03185 and SGL_RS03190 are separated by 18 nucleotides SGL_RS03190 and SGL_RS03195 are separated by 63 nucleotides SGL_RS03195 and SGL_RS19760 are separated by 66 nucleotides SGL_RS19760 and SGL_RS19765 overlap by 32 nucleotides SGL_RS19765 and SGL_RS03210 are separated by 238 nucleotides
SGL_RS03185: SGL_RS03185 - biotin--[acetyl-CoA-carboxylase] ligase, at 280,127 to 280,951
_RS03185
SGL_RS03190: SGL_RS03190 - universal stress protein, at 280,970 to 281,443
_RS03190
SGL_RS03195: SGL_RS03195 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 281,507 to 282,223
_RS03195
SGL_RS19760: SGL_RS19760 - CopG family antitoxin, at 282,290 to 282,541
_RS19760
SGL_RS19765: SGL_RS19765 - BrnT family toxin, at 282,510 to 282,821
_RS19765
SGL_RS03210: SGL_RS03210 - NADP-dependent isocitrate dehydrogenase, at 283,060 to 284,487
_RS03210
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 280.579 kb on + strand, within SGL_RS03185 at 280.633 kb on - strand, within SGL_RS03185 at 280.644 kb on + strand, within SGL_RS03185 at 280.706 kb on + strand, within SGL_RS03185 at 280.840 kb on - strand, within SGL_RS03185 at 280.849 kb on - strand, within SGL_RS03185 at 280.875 kb on + strand at 280.876 kb on - strand at 280.926 kb on + strand at 280.934 kb on - strand at 280.976 kb on - strand at 280.976 kb on - strand at 280.987 kb on - strand at 280.992 kb on - strand at 280.992 kb on - strand at 281.019 kb on - strand, within SGL_RS03190 at 281.043 kb on - strand, within SGL_RS03190 at 281.321 kb on - strand, within SGL_RS03190 at 281.326 kb on - strand, within SGL_RS03190 at 281.326 kb on - strand, within SGL_RS03190 at 281.399 kb on + strand at 281.399 kb on - strand at 281.531 kb on + strand at 281.562 kb on + strand at 281.618 kb on - strand, within SGL_RS03195 at 281.643 kb on - strand, within SGL_RS03195 at 281.681 kb on - strand, within SGL_RS03195 at 281.691 kb on - strand, within SGL_RS03195 at 281.860 kb on - strand, within SGL_RS03195 at 281.873 kb on + strand, within SGL_RS03195 at 282.044 kb on + strand, within SGL_RS03195 at 282.070 kb on + strand, within SGL_RS03195 at 282.174 kb on - strand at 282.563 kb on + strand, within SGL_RS19765 at 282.573 kb on - strand, within SGL_RS19765 at 282.664 kb on - strand, within SGL_RS19765 at 282.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 280,579 + SGL_RS03185 0.55 +0.9 280,633 - SGL_RS03185 0.61 +1.2 280,644 + SGL_RS03185 0.63 +0.6 280,706 + SGL_RS03185 0.70 -0.1 280,840 - SGL_RS03185 0.86 -0.6 280,849 - SGL_RS03185 0.88 -1.2 280,875 + +1.2 280,876 - +0.6 280,926 + -2.2 280,934 - -0.2 280,976 - -1.5 280,976 - -0.8 280,987 - +4.8 280,992 - -1.8 280,992 - +1.0 281,019 - SGL_RS03190 0.10 +2.2 281,043 - SGL_RS03190 0.15 -0.9 281,321 - SGL_RS03190 0.74 +0.7 281,326 - SGL_RS03190 0.75 -2.5 281,326 - SGL_RS03190 0.75 +0.2 281,399 + -2.1 281,399 - -0.4 281,531 + -0.9 281,562 + +0.4 281,618 - SGL_RS03195 0.15 -1.7 281,643 - SGL_RS03195 0.19 -1.8 281,681 - SGL_RS03195 0.24 -2.2 281,691 - SGL_RS03195 0.26 -2.6 281,860 - SGL_RS03195 0.49 -1.6 281,873 + SGL_RS03195 0.51 -0.6 282,044 + SGL_RS03195 0.75 -0.0 282,070 + SGL_RS03195 0.79 -1.7 282,174 - -2.0 282,563 + SGL_RS19765 0.17 +1.9 282,573 - SGL_RS19765 0.20 -1.2 282,664 - SGL_RS19765 0.49 +0.5 282,879 - +1.6
Or see this region's nucleotide sequence