Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02515 and SGL_RS02520 are separated by 234 nucleotides SGL_RS02520 and SGL_RS02525 are separated by 10 nucleotides SGL_RS02525 and SGL_RS02530 are separated by 82 nucleotides
SGL_RS02515: SGL_RS02515 - M56 family metallopeptidase, at 142,981 to 143,826
_RS02515
SGL_RS02520: SGL_RS02520 - thioredoxin-dependent thiol peroxidase, at 144,061 to 144,543
_RS02520
SGL_RS02525: SGL_RS02525 - hypothetical protein, at 144,554 to 145,081
_RS02525
SGL_RS02530: SGL_RS02530 - MTH1187 family thiamine-binding protein, at 145,164 to 145,529
_RS02530
Position (kb) 144
145 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 143.210 kb on + strand, within SGL_RS02515 at 143.343 kb on + strand, within SGL_RS02515 at 143.357 kb on + strand, within SGL_RS02515 at 143.419 kb on + strand, within SGL_RS02515 at 143.543 kb on + strand, within SGL_RS02515 at 143.664 kb on + strand, within SGL_RS02515 at 143.927 kb on + strand at 144.473 kb on - strand, within SGL_RS02520 at 144.531 kb on - strand at 144.693 kb on - strand, within SGL_RS02525 at 144.766 kb on + strand, within SGL_RS02525 at 144.821 kb on + strand, within SGL_RS02525 at 145.410 kb on - strand, within SGL_RS02530 at 145.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 143,210 + SGL_RS02515 0.27 +1.2 143,343 + SGL_RS02515 0.43 -1.6 143,357 + SGL_RS02515 0.44 +0.2 143,419 + SGL_RS02515 0.52 +0.3 143,543 + SGL_RS02515 0.66 +0.9 143,664 + SGL_RS02515 0.81 -0.2 143,927 + +2.3 144,473 - SGL_RS02520 0.85 -3.4 144,531 - -1.5 144,693 - SGL_RS02525 0.26 +0.1 144,766 + SGL_RS02525 0.40 -1.5 144,821 + SGL_RS02525 0.51 -3.9 145,410 - SGL_RS02530 0.67 +0.3 145,499 - -0.4
Or see this region's nucleotide sequence