Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12240 and SGL_RS12245 overlap by 56 nucleotides SGL_RS12245 and SGL_RS18950 are separated by 66 nucleotides SGL_RS18950 and SGL_RS18955 are separated by 223 nucleotides SGL_RS18955 and SGL_RS12255 are separated by 120 nucleotides
SGL_RS12240: SGL_RS12240 - S-methyl-5'-thioadenosine phosphorylase, at 2,208,778 to 2,209,758
_RS12240
SGL_RS12245: SGL_RS12245 - DUF3134 domain-containing protein, at 2,209,703 to 2,209,939
_RS12245
SGL_RS18950: SGL_RS18950 - ribbon-helix-helix domain-containing protein, at 2,210,006 to 2,210,161
_RS18950
SGL_RS18955: SGL_RS18955 - IS5 family transposase, at 2,210,385 to 2,211,162
_RS18955
SGL_RS12255: SGL_RS12255 - Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain, at 2,211,283 to 2,212,479
_RS12255
Position (kb) 2210
2211
2212 Strain fitness (log2 ratio) -1
0
1
2 at 2209.464 kb on + strand, within SGL_RS12240 at 2209.550 kb on - strand, within SGL_RS12240 at 2211.066 kb on + strand, within SGL_RS18955 at 2211.101 kb on - strand at 2212.136 kb on + strand, within SGL_RS12255
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 2,209,464 + SGL_RS12240 0.70 -0.5 2,209,550 - SGL_RS12240 0.79 +1.3 2,211,066 + SGL_RS18955 0.88 +0.9 2,211,101 - +1.9 2,212,136 + SGL_RS12255 0.71 -0.8
Or see this region's nucleotide sequence