Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15040 and SGL_RS15045 are separated by 110 nucleotides SGL_RS15045 and SGL_RS15050 are separated by 129 nucleotides SGL_RS15050 and SGL_RS15055 overlap by 25 nucleotides SGL_RS15055 and SGL_RS15060 are separated by 6 nucleotides
SGL_RS15040: SGL_RS15040 - acyl-CoA desaturase, at 2,822,579 to 2,823,535
_RS15040
SGL_RS15045: SGL_RS15045 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 2,823,646 to 2,824,893
_RS15045
SGL_RS15050: SGL_RS15050 - MliC family protein, at 2,825,023 to 2,825,355
_RS15050
SGL_RS15055: SGL_RS15055 - hypothetical protein, at 2,825,331 to 2,825,711
_RS15055
SGL_RS15060: SGL_RS15060 - PstS family phosphate ABC transporter substrate-binding protein, at 2,825,718 to 2,826,641
_RS15060
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2823.780 kb on - strand, within SGL_RS15045 at 2823.789 kb on + strand, within SGL_RS15045 at 2823.797 kb on + strand, within SGL_RS15045 at 2823.857 kb on - strand, within SGL_RS15045 at 2823.857 kb on - strand, within SGL_RS15045 at 2823.904 kb on + strand, within SGL_RS15045 at 2823.916 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.970 kb on + strand, within SGL_RS15045 at 2823.978 kb on - strand, within SGL_RS15045 at 2823.978 kb on - strand, within SGL_RS15045 at 2824.046 kb on - strand, within SGL_RS15045 at 2824.090 kb on + strand, within SGL_RS15045 at 2824.165 kb on + strand, within SGL_RS15045 at 2824.358 kb on - strand, within SGL_RS15045 at 2824.384 kb on - strand, within SGL_RS15045 at 2824.513 kb on - strand, within SGL_RS15045 at 2824.649 kb on - strand, within SGL_RS15045 at 2824.724 kb on - strand, within SGL_RS15045 at 2824.731 kb on - strand, within SGL_RS15045 at 2824.785 kb on + strand at 2824.824 kb on - strand at 2824.925 kb on - strand at 2824.935 kb on + strand at 2825.224 kb on + strand, within SGL_RS15050 at 2825.231 kb on - strand, within SGL_RS15050 at 2825.287 kb on - strand, within SGL_RS15050 at 2825.495 kb on + strand, within SGL_RS15055 at 2825.522 kb on + strand, within SGL_RS15055 at 2825.537 kb on - strand, within SGL_RS15055 at 2825.728 kb on + strand at 2825.798 kb on - strand at 2825.863 kb on - strand, within SGL_RS15060
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 2,823,780 - SGL_RS15045 0.11 -4.2 2,823,789 + SGL_RS15045 0.11 -2.4 2,823,797 + SGL_RS15045 0.12 -4.9 2,823,857 - SGL_RS15045 0.17 -0.3 2,823,857 - SGL_RS15045 0.17 -4.4 2,823,904 + SGL_RS15045 0.21 -3.8 2,823,916 + SGL_RS15045 0.22 -5.2 2,823,970 + SGL_RS15045 0.26 -4.9 2,823,970 + SGL_RS15045 0.26 -0.8 2,823,970 + SGL_RS15045 0.26 -2.4 2,823,978 - SGL_RS15045 0.27 -3.6 2,823,978 - SGL_RS15045 0.27 -1.6 2,824,046 - SGL_RS15045 0.32 -1.9 2,824,090 + SGL_RS15045 0.36 -2.3 2,824,165 + SGL_RS15045 0.42 -1.8 2,824,358 - SGL_RS15045 0.57 -3.4 2,824,384 - SGL_RS15045 0.59 -1.6 2,824,513 - SGL_RS15045 0.69 -4.7 2,824,649 - SGL_RS15045 0.80 -4.6 2,824,724 - SGL_RS15045 0.86 -4.8 2,824,731 - SGL_RS15045 0.87 -3.0 2,824,785 + -4.2 2,824,824 - -2.2 2,824,925 - +0.8 2,824,935 + -0.3 2,825,224 + SGL_RS15050 0.60 -0.8 2,825,231 - SGL_RS15050 0.62 +0.5 2,825,287 - SGL_RS15050 0.79 -1.3 2,825,495 + SGL_RS15055 0.43 -0.3 2,825,522 + SGL_RS15055 0.50 -0.5 2,825,537 - SGL_RS15055 0.54 +0.5 2,825,728 + +0.2 2,825,798 - -0.8 2,825,863 - SGL_RS15060 0.16 -2.8
Or see this region's nucleotide sequence