Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14500 and SGL_RS14505 are separated by 77 nucleotides SGL_RS14505 and SGL_RS14510 are separated by 125 nucleotides SGL_RS14510 and SGL_RS14515 are separated by 135 nucleotides
SGL_RS14500: SGL_RS14500 - urea ABC transporter ATP-binding subunit UrtE, at 2,708,257 to 2,709,006
_RS14500
SGL_RS14505: SGL_RS14505 - glutamate-5-semialdehyde dehydrogenase, at 2,709,084 to 2,710,385
_RS14505
SGL_RS14510: SGL_RS14510 - DUF3177 family protein, at 2,710,511 to 2,711,107
_RS14510
SGL_RS14515: SGL_RS14515 - type II toxin-antitoxin system HicA family toxin, at 2,711,243 to 2,711,422
_RS14515
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2708.231 kb on - strand at 2708.291 kb on - strand at 2708.513 kb on + strand, within SGL_RS14500 at 2708.674 kb on + strand, within SGL_RS14500 at 2708.740 kb on - strand, within SGL_RS14500 at 2708.823 kb on - strand, within SGL_RS14500 at 2708.924 kb on + strand, within SGL_RS14500 at 2708.982 kb on - strand at 2709.119 kb on + strand at 2709.141 kb on - strand at 2709.164 kb on + strand at 2709.172 kb on - strand at 2709.388 kb on - strand, within SGL_RS14505 at 2709.461 kb on - strand, within SGL_RS14505 at 2709.540 kb on + strand, within SGL_RS14505 at 2709.554 kb on - strand, within SGL_RS14505 at 2709.649 kb on + strand, within SGL_RS14505 at 2709.671 kb on - strand, within SGL_RS14505 at 2709.745 kb on - strand, within SGL_RS14505 at 2710.087 kb on - strand, within SGL_RS14505 at 2710.178 kb on - strand, within SGL_RS14505 at 2710.380 kb on + strand at 2710.439 kb on - strand at 2710.489 kb on + strand at 2710.497 kb on - strand at 2710.650 kb on + strand, within SGL_RS14510 at 2710.652 kb on + strand, within SGL_RS14510 at 2710.660 kb on - strand, within SGL_RS14510 at 2710.722 kb on - strand, within SGL_RS14510 at 2711.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 2,708,231 - -0.1 2,708,291 - -0.6 2,708,513 + SGL_RS14500 0.34 -1.4 2,708,674 + SGL_RS14500 0.56 +0.3 2,708,740 - SGL_RS14500 0.64 +0.8 2,708,823 - SGL_RS14500 0.75 -0.1 2,708,924 + SGL_RS14500 0.89 -1.0 2,708,982 - -0.3 2,709,119 + +1.8 2,709,141 - -3.1 2,709,164 + -3.8 2,709,172 - -4.1 2,709,388 - SGL_RS14505 0.23 -4.2 2,709,461 - SGL_RS14505 0.29 -3.4 2,709,540 + SGL_RS14505 0.35 -5.7 2,709,554 - SGL_RS14505 0.36 -4.3 2,709,649 + SGL_RS14505 0.43 -2.2 2,709,671 - SGL_RS14505 0.45 -3.4 2,709,745 - SGL_RS14505 0.51 -2.8 2,710,087 - SGL_RS14505 0.77 -2.0 2,710,178 - SGL_RS14505 0.84 -3.3 2,710,380 + +0.6 2,710,439 - -0.8 2,710,489 + +0.1 2,710,497 - -0.8 2,710,650 + SGL_RS14510 0.23 -0.3 2,710,652 + SGL_RS14510 0.24 -2.0 2,710,660 - SGL_RS14510 0.25 +0.7 2,710,722 - SGL_RS14510 0.35 +2.0 2,711,162 + -0.9
Or see this region's nucleotide sequence