Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12350 and SGL_RS12355 are separated by 0 nucleotides SGL_RS12355 and SGL_RS12360 overlap by 26 nucleotides SGL_RS12360 and SGL_RS12365 are separated by 30 nucleotides SGL_RS12365 and SGL_RS12370 are separated by 224 nucleotides SGL_RS12370 and SGL_RS12375 are separated by 21 nucleotides
SGL_RS12350: SGL_RS12350 - MFS transporter, at 2,231,639 to 2,233,036
_RS12350
SGL_RS12355: SGL_RS12355 - exodeoxyribonuclease III, at 2,233,037 to 2,233,834
_RS12355
SGL_RS12360: SGL_RS12360 - DUF3750 domain-containing protein, at 2,233,809 to 2,234,273
_RS12360
SGL_RS12365: SGL_RS12365 - nucleoside-diphosphate kinase, at 2,234,304 to 2,234,753
_RS12365
SGL_RS12370: SGL_RS12370 - tRNA-Ala, at 2,234,978 to 2,235,050
_RS12370
SGL_RS12375: SGL_RS12375 - hypothetical protein, at 2,235,072 to 2,235,482
_RS12375
Position (kb)
2233
2234
2235 Strain fitness (log2 ratio)
-2
-1
0
1 at 2232.920 kb on + strand at 2233.372 kb on + strand, within SGL_RS12355 at 2233.417 kb on - strand, within SGL_RS12355 at 2233.475 kb on + strand, within SGL_RS12355 at 2233.475 kb on + strand, within SGL_RS12355 at 2233.529 kb on - strand, within SGL_RS12355 at 2233.564 kb on - strand, within SGL_RS12355 at 2233.646 kb on + strand, within SGL_RS12355 at 2233.868 kb on + strand, within SGL_RS12360 at 2233.869 kb on - strand, within SGL_RS12360 at 2233.969 kb on + strand, within SGL_RS12360 at 2234.116 kb on + strand, within SGL_RS12360 at 2234.158 kb on - strand, within SGL_RS12360 at 2234.328 kb on - strand at 2234.365 kb on - strand, within SGL_RS12365 at 2234.411 kb on + strand, within SGL_RS12365 at 2234.540 kb on - strand, within SGL_RS12365 at 2234.867 kb on - strand at 2234.959 kb on + strand at 2235.159 kb on + strand, within SGL_RS12375
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 2,232,920 + -0.2 2,233,372 + SGL_RS12355 0.42 +1.1 2,233,417 - SGL_RS12355 0.48 -0.8 2,233,475 + SGL_RS12355 0.55 +0.9 2,233,475 + SGL_RS12355 0.55 +0.6 2,233,529 - SGL_RS12355 0.62 -0.2 2,233,564 - SGL_RS12355 0.66 +0.6 2,233,646 + SGL_RS12355 0.76 +1.5 2,233,868 + SGL_RS12360 0.13 +1.1 2,233,869 - SGL_RS12360 0.13 +0.3 2,233,969 + SGL_RS12360 0.34 +1.1 2,234,116 + SGL_RS12360 0.66 +1.0 2,234,158 - SGL_RS12360 0.75 +1.4 2,234,328 - +1.2 2,234,365 - SGL_RS12365 0.14 -2.6 2,234,411 + SGL_RS12365 0.24 -2.2 2,234,540 - SGL_RS12365 0.52 -1.8 2,234,867 - -0.4 2,234,959 + +0.6 2,235,159 + SGL_RS12375 0.21 +0.1
Or see this region's nucleotide sequence