Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02295 and SGL_RS02300 are separated by 127 nucleotides SGL_RS02300 and SGL_RS02305 are separated by 202 nucleotides SGL_RS02305 and SGL_RS02310 are separated by 0 nucleotides
SGL_RS02295: SGL_RS02295 - polyribonucleotide nucleotidyltransferase, at 96,325 to 98,481
_RS02295
SGL_RS02300: SGL_RS02300 - signal peptidase I, at 98,609 to 99,199
_RS02300
SGL_RS02305: SGL_RS02305 - NAD-dependent malic enzyme, at 99,402 to 100,793
_RS02305
SGL_RS02310: SGL_RS02310 - cysteine synthase A, at 100,794 to 101,771
_RS02310
Position (kb) 99
100
101 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 98.547 kb on + strand at 98.550 kb on - strand at 98.633 kb on - strand at 98.638 kb on - strand at 98.648 kb on - strand at 98.795 kb on - strand, within SGL_RS02300 at 98.945 kb on + strand, within SGL_RS02300 at 99.143 kb on - strand at 99.230 kb on + strand at 99.272 kb on + strand at 99.306 kb on + strand at 99.405 kb on - strand at 99.415 kb on + strand at 99.433 kb on + strand at 99.533 kb on + strand at 99.552 kb on - strand, within SGL_RS02305 at 99.618 kb on + strand, within SGL_RS02305 at 99.632 kb on - strand, within SGL_RS02305 at 99.743 kb on + strand, within SGL_RS02305 at 99.748 kb on - strand, within SGL_RS02305 at 99.749 kb on - strand, within SGL_RS02305 at 99.895 kb on - strand, within SGL_RS02305 at 100.009 kb on + strand, within SGL_RS02305 at 100.118 kb on + strand, within SGL_RS02305 at 100.121 kb on - strand, within SGL_RS02305 at 100.208 kb on - strand, within SGL_RS02305 at 100.229 kb on - strand, within SGL_RS02305 at 100.354 kb on - strand, within SGL_RS02305 at 100.439 kb on + strand, within SGL_RS02305 at 100.467 kb on + strand, within SGL_RS02305 at 100.472 kb on + strand, within SGL_RS02305 at 100.480 kb on - strand, within SGL_RS02305 at 100.676 kb on - strand at 100.678 kb on + strand at 100.684 kb on - strand at 100.691 kb on - strand at 101.089 kb on + strand, within SGL_RS02310 at 101.130 kb on - strand, within SGL_RS02310 at 101.139 kb on - strand, within SGL_RS02310 at 101.149 kb on - strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.179 kb on + strand, within SGL_RS02310 at 101.187 kb on - strand, within SGL_RS02310 at 101.212 kb on - strand, within SGL_RS02310 at 101.218 kb on - strand, within SGL_RS02310 at 101.220 kb on + strand, within SGL_RS02310 at 101.308 kb on + strand, within SGL_RS02310 at 101.358 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.363 kb on - strand, within SGL_RS02310 at 101.415 kb on - strand, within SGL_RS02310 at 101.428 kb on + strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.436 kb on - strand, within SGL_RS02310 at 101.449 kb on + strand, within SGL_RS02310 at 101.505 kb on + strand, within SGL_RS02310 at 101.735 kb on + strand at 101.781 kb on + strand at 101.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 98,547 + +0.3 98,550 - -0.4 98,633 - -0.5 98,638 - +0.3 98,648 - -0.3 98,795 - SGL_RS02300 0.31 -1.9 98,945 + SGL_RS02300 0.57 -0.9 99,143 - -1.3 99,230 + +0.4 99,272 + +1.1 99,306 + +0.2 99,405 - -5.5 99,415 + -4.1 99,433 + -4.2 99,533 + -1.9 99,552 - SGL_RS02305 0.11 -5.2 99,618 + SGL_RS02305 0.16 -2.6 99,632 - SGL_RS02305 0.17 -2.6 99,743 + SGL_RS02305 0.24 -5.9 99,748 - SGL_RS02305 0.25 -4.5 99,749 - SGL_RS02305 0.25 -5.9 99,895 - SGL_RS02305 0.35 -2.3 100,009 + SGL_RS02305 0.44 -5.3 100,118 + SGL_RS02305 0.51 -1.8 100,121 - SGL_RS02305 0.52 -4.9 100,208 - SGL_RS02305 0.58 -4.7 100,229 - SGL_RS02305 0.59 -4.5 100,354 - SGL_RS02305 0.68 -5.7 100,439 + SGL_RS02305 0.74 -5.4 100,467 + SGL_RS02305 0.77 -5.3 100,472 + SGL_RS02305 0.77 -3.2 100,480 - SGL_RS02305 0.77 -3.7 100,676 - -1.6 100,678 + -4.7 100,684 - -2.6 100,691 - -3.9 101,089 + SGL_RS02310 0.30 +0.3 101,130 - SGL_RS02310 0.34 +0.6 101,139 - SGL_RS02310 0.35 -0.3 101,149 - SGL_RS02310 0.36 +0.0 101,179 + SGL_RS02310 0.39 +1.2 101,179 + SGL_RS02310 0.39 +0.6 101,187 - SGL_RS02310 0.40 +1.1 101,212 - SGL_RS02310 0.43 +1.4 101,218 - SGL_RS02310 0.43 +0.9 101,220 + SGL_RS02310 0.44 +0.5 101,308 + SGL_RS02310 0.53 -0.3 101,358 - SGL_RS02310 0.58 +0.6 101,363 - SGL_RS02310 0.58 -0.0 101,363 - SGL_RS02310 0.58 +0.8 101,415 - SGL_RS02310 0.63 +0.8 101,428 + SGL_RS02310 0.65 +0.8 101,436 - SGL_RS02310 0.66 +0.8 101,436 - SGL_RS02310 0.66 +0.9 101,449 + SGL_RS02310 0.67 +0.8 101,505 + SGL_RS02310 0.73 +0.4 101,735 + +0.8 101,781 + -0.4 101,781 + -1.1
Or see this region's nucleotide sequence