Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06770 and SGL_RS06775 are separated by 48 nucleotides SGL_RS06775 and SGL_RS06780 overlap by 4 nucleotides SGL_RS06780 and SGL_RS06785 are separated by 500 nucleotides
SGL_RS06770: SGL_RS06770 - cytosine deaminase, at 1,047,005 to 1,048,357
_RS06770
SGL_RS06775: SGL_RS06775 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,048,406 to 1,048,753
_RS06775
SGL_RS06780: SGL_RS06780 - hypothetical protein, at 1,048,750 to 1,049,016
_RS06780
SGL_RS06785: SGL_RS06785 - alpha/beta hydrolase, at 1,049,517 to 1,050,407
_RS06785
Position (kb)
1048
1049
1050 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1047.840 kb on + strand, within SGL_RS06770 at 1047.848 kb on - strand, within SGL_RS06770 at 1047.895 kb on + strand, within SGL_RS06770 at 1047.931 kb on + strand, within SGL_RS06770 at 1048.109 kb on + strand, within SGL_RS06770 at 1048.339 kb on - strand at 1048.403 kb on + strand at 1048.456 kb on - strand, within SGL_RS06775 at 1048.564 kb on - strand, within SGL_RS06775 at 1048.615 kb on + strand, within SGL_RS06775 at 1048.854 kb on + strand, within SGL_RS06780 at 1048.861 kb on + strand, within SGL_RS06780 at 1048.862 kb on - strand, within SGL_RS06780 at 1048.935 kb on + strand, within SGL_RS06780 at 1049.024 kb on - strand at 1049.026 kb on - strand at 1049.212 kb on + strand at 1049.641 kb on - strand, within SGL_RS06785 at 1049.751 kb on + strand, within SGL_RS06785 at 1049.763 kb on + strand, within SGL_RS06785 at 1049.794 kb on + strand, within SGL_RS06785 at 1049.794 kb on + strand, within SGL_RS06785 at 1049.841 kb on + strand, within SGL_RS06785 at 1049.864 kb on + strand, within SGL_RS06785 at 1049.872 kb on - strand, within SGL_RS06785 at 1049.945 kb on + strand, within SGL_RS06785 at 1050.011 kb on - strand, within SGL_RS06785
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,047,840 + SGL_RS06770 0.62 -0.4 1,047,848 - SGL_RS06770 0.62 -0.4 1,047,895 + SGL_RS06770 0.66 -0.0 1,047,931 + SGL_RS06770 0.68 -0.0 1,048,109 + SGL_RS06770 0.82 -0.7 1,048,339 - -3.7 1,048,403 + -0.8 1,048,456 - SGL_RS06775 0.14 -3.4 1,048,564 - SGL_RS06775 0.45 -2.3 1,048,615 + SGL_RS06775 0.60 -1.5 1,048,854 + SGL_RS06780 0.39 -3.0 1,048,861 + SGL_RS06780 0.42 -3.9 1,048,862 - SGL_RS06780 0.42 -4.2 1,048,935 + SGL_RS06780 0.69 -3.4 1,049,024 - -3.2 1,049,026 - -2.6 1,049,212 + +0.9 1,049,641 - SGL_RS06785 0.14 +0.2 1,049,751 + SGL_RS06785 0.26 -0.2 1,049,763 + SGL_RS06785 0.28 -0.0 1,049,794 + SGL_RS06785 0.31 -0.7 1,049,794 + SGL_RS06785 0.31 +0.0 1,049,841 + SGL_RS06785 0.36 -1.7 1,049,864 + SGL_RS06785 0.39 -0.9 1,049,872 - SGL_RS06785 0.40 -0.3 1,049,945 + SGL_RS06785 0.48 +0.5 1,050,011 - SGL_RS06785 0.55 -0.5
Or see this region's nucleotide sequence