Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07180 and SGL_RS18765 are separated by 157 nucleotides SGL_RS18765 and SGL_RS07185 are separated by 154 nucleotides SGL_RS07185 and SGL_RS19815 are separated by 88 nucleotides SGL_RS19815 and SGL_RS19820 overlap by 2 nucleotides
SGL_RS07180: SGL_RS07180 - carboxynorspermidine decarboxylase, at 1,140,497 to 1,141,645
_RS07180
SGL_RS18765: SGL_RS18765 - chlorophyll A-B-binding protein, at 1,141,803 to 1,141,946
_RS18765
SGL_RS07185: SGL_RS07185 - hypothetical protein, at 1,142,101 to 1,142,412
_RS07185
SGL_RS19815: SGL_RS19815 - hypothetical protein, at 1,142,501 to 1,142,707
_RS19815
SGL_RS19820: SGL_RS19820 - hypothetical protein, at 1,142,706 to 1,142,864
_RS19820
Position (kb)
1141
1142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 1140.997 kb on - strand, within SGL_RS07180 at 1141.026 kb on + strand, within SGL_RS07180 at 1141.145 kb on - strand, within SGL_RS07180 at 1141.325 kb on - strand, within SGL_RS07180 at 1141.457 kb on + strand, within SGL_RS07180 at 1141.516 kb on + strand, within SGL_RS07180 at 1141.525 kb on - strand, within SGL_RS07180 at 1141.701 kb on - strand at 1141.854 kb on + strand, within SGL_RS18765 at 1141.949 kb on - strand at 1142.065 kb on - strand at 1142.099 kb on - strand at 1142.099 kb on - strand at 1142.137 kb on + strand, within SGL_RS07185 at 1142.165 kb on - strand, within SGL_RS07185 at 1142.165 kb on - strand, within SGL_RS07185 at 1142.185 kb on + strand, within SGL_RS07185
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,140,997 - SGL_RS07180 0.44 +2.9 1,141,026 + SGL_RS07180 0.46 +5.3 1,141,145 - SGL_RS07180 0.56 +8.1 1,141,325 - SGL_RS07180 0.72 +5.2 1,141,457 + SGL_RS07180 0.84 +6.4 1,141,516 + SGL_RS07180 0.89 +4.9 1,141,525 - SGL_RS07180 0.89 +4.6 1,141,701 - -1.0 1,141,854 + SGL_RS18765 0.35 -2.6 1,141,949 - -0.8 1,142,065 - -1.2 1,142,099 - -1.4 1,142,099 - +0.2 1,142,137 + SGL_RS07185 0.12 +0.3 1,142,165 - SGL_RS07185 0.21 +0.4 1,142,165 - SGL_RS07185 0.21 -2.9 1,142,185 + SGL_RS07185 0.27 -1.5
Or see this region's nucleotide sequence