Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18335 and SGL_RS18340 are separated by 17 nucleotides SGL_RS18340 and SGL_RS18345 are separated by 280 nucleotides SGL_RS18345 and SGL_RS20050 are separated by 5 nucleotides SGL_RS20050 and SGL_RS18350 are separated by 25 nucleotides
SGL_RS18335: SGL_RS18335 - hypothetical protein, at 3,560,755 to 3,561,144
_RS18335
SGL_RS18340: SGL_RS18340 - hypothetical protein, at 3,561,162 to 3,561,665
_RS18340
SGL_RS18345: SGL_RS18345 - trans-splicing intein-formed DNA polymerase III subunit alpha N-terminal partner DnaE-N, at 3,561,946 to 3,564,639
_RS18345
SGL_RS20050: SGL_RS20050 - hypothetical protein, at 3,564,645 to 3,564,812
_RS20050
SGL_RS18350: SGL_RS18350 - translation elongation factor 4, at 3,564,838 to 3,566,649
_RS18350
Position (kb) 3561
3562
3563
3564
3565 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9
10
11
12 at 3560.957 kb on + strand, within SGL_RS18335 at 3561.372 kb on + strand, within SGL_RS18340 at 3564.591 kb on - strand at 3564.693 kb on - strand, within SGL_RS20050 at 3565.025 kb on - strand, within SGL_RS18350 at 3565.052 kb on - strand, within SGL_RS18350 at 3565.053 kb on - strand, within SGL_RS18350 at 3565.132 kb on - strand, within SGL_RS18350 at 3565.191 kb on + strand, within SGL_RS18350 at 3565.197 kb on + strand, within SGL_RS18350
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 3,560,957 + SGL_RS18335 0.52 +0.3 3,561,372 + SGL_RS18340 0.42 -0.2 3,564,591 - -1.3 3,564,693 - SGL_RS20050 0.29 +0.1 3,565,025 - SGL_RS18350 0.10 +10.0 3,565,052 - SGL_RS18350 0.12 +10.4 3,565,053 - SGL_RS18350 0.12 +9.6 3,565,132 - SGL_RS18350 0.16 +12.5 3,565,191 + SGL_RS18350 0.19 +9.6 3,565,197 + SGL_RS18350 0.20 +9.6
Or see this region's nucleotide sequence