Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17050 and SGL_RS17055 are separated by 71 nucleotides SGL_RS17055 and SGL_RS17060 are separated by 21 nucleotides SGL_RS17060 and SGL_RS17065 are separated by 134 nucleotides
SGL_RS17050: SGL_RS17050 - UDP-N-acetylmuramate--L-alanine ligase, at 3,279,390 to 3,280,907
_RS17050
SGL_RS17055: SGL_RS17055 - UDP-N-acetylmuramate dehydrogenase, at 3,280,979 to 3,281,932
_RS17055
SGL_RS17060: SGL_RS17060 - hypothetical protein, at 3,281,954 to 3,282,307
_RS17060
SGL_RS17065: SGL_RS17065 - bacterioferritin, at 3,282,442 to 3,282,912
_RS17065
Position (kb)
3280
3281
3282 Strain fitness (log2 ratio)
-2
-1
0
1 at 3281.039 kb on + strand at 3281.986 kb on - strand at 3282.023 kb on + strand, within SGL_RS17060 at 3282.025 kb on - strand, within SGL_RS17060 at 3282.032 kb on + strand, within SGL_RS17060 at 3282.037 kb on + strand, within SGL_RS17060 at 3282.037 kb on - strand, within SGL_RS17060 at 3282.126 kb on - strand, within SGL_RS17060 at 3282.330 kb on - strand at 3282.342 kb on - strand at 3282.536 kb on - strand, within SGL_RS17065 at 3282.574 kb on + strand, within SGL_RS17065 at 3282.616 kb on + strand, within SGL_RS17065 at 3282.633 kb on + strand, within SGL_RS17065 at 3282.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 3,281,039 + -2.6 3,281,986 - +0.7 3,282,023 + SGL_RS17060 0.19 -1.0 3,282,025 - SGL_RS17060 0.20 -2.3 3,282,032 + SGL_RS17060 0.22 -0.2 3,282,037 + SGL_RS17060 0.23 -0.5 3,282,037 - SGL_RS17060 0.23 -1.7 3,282,126 - SGL_RS17060 0.49 +0.5 3,282,330 - +0.4 3,282,342 - +0.4 3,282,536 - SGL_RS17065 0.20 +0.4 3,282,574 + SGL_RS17065 0.28 +0.9 3,282,616 + SGL_RS17065 0.37 +0.6 3,282,633 + SGL_RS17065 0.41 -2.0 3,282,889 - +0.3
Or see this region's nucleotide sequence