Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14975 and SGL_RS14980 are separated by 193 nucleotides SGL_RS14980 and SGL_RS14985 are separated by 59 nucleotides SGL_RS14985 and SGL_RS14990 are separated by 74 nucleotides SGL_RS14990 and SGL_RS14995 are separated by 198 nucleotides SGL_RS14995 and SGL_RS15000 are separated by 57 nucleotides
SGL_RS14975: SGL_RS14975 - translation initiation factor, at 2,812,385 to 2,812,720
_RS14975
SGL_RS14980: SGL_RS14980 - phosphatase PAP2 family protein, at 2,812,914 to 2,813,594
_RS14980
SGL_RS14985: SGL_RS14985 - DNA polymerase III subunit delta, at 2,813,654 to 2,814,655
_RS14985
SGL_RS14990: SGL_RS14990 - hypothetical protein, at 2,814,730 to 2,814,924
_RS14990
SGL_RS14995: SGL_RS14995 - hypothetical protein, at 2,815,123 to 2,815,509
_RS14995
SGL_RS15000: SGL_RS15000 - DUF2752 domain-containing protein, at 2,815,567 to 2,816,013
_RS15000
Position (kb)
2813
2814
2815 Strain fitness (log2 ratio)
-1
0
1 at 2812.715 kb on + strand at 2812.723 kb on - strand at 2812.723 kb on - strand at 2814.655 kb on + strand at 2814.692 kb on + strand at 2814.694 kb on - strand at 2814.700 kb on - strand at 2814.778 kb on + strand, within SGL_RS14990 at 2814.789 kb on + strand, within SGL_RS14990 at 2814.797 kb on - strand, within SGL_RS14990 at 2814.802 kb on - strand, within SGL_RS14990 at 2814.802 kb on - strand, within SGL_RS14990 at 2814.928 kb on + strand at 2814.951 kb on - strand at 2815.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,812,715 + -0.7 2,812,723 - +0.7 2,812,723 - +0.5 2,814,655 + -1.0 2,814,692 + +1.3 2,814,694 - -1.2 2,814,700 - +0.5 2,814,778 + SGL_RS14990 0.25 -0.3 2,814,789 + SGL_RS14990 0.30 +0.8 2,814,797 - SGL_RS14990 0.34 -0.9 2,814,802 - SGL_RS14990 0.37 -0.8 2,814,802 - SGL_RS14990 0.37 -0.3 2,814,928 + -0.5 2,814,951 - -0.2 2,815,002 - +1.1
Or see this region's nucleotide sequence