Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10950 and SGL_RS10955 are separated by 204 nucleotides SGL_RS10955 and SGL_RS10960 are separated by 52 nucleotides SGL_RS10960 and SGL_RS19900 are separated by 55 nucleotides SGL_RS19900 and SGL_RS19905 are separated by 152 nucleotides
SGL_RS10950: SGL_RS10950 - phosphoribosylamine--glycine ligase, at 1,920,660 to 1,921,919
_RS10950
SGL_RS10955: SGL_RS10955 - hypothetical protein, at 1,922,124 to 1,922,738
_RS10955
SGL_RS10960: SGL_RS10960 - RpiB/LacA/LacB family sugar-phosphate isomerase, at 1,922,791 to 1,923,114
_RS10960
SGL_RS19900: SGL_RS19900 - transposase, at 1,923,170 to 1,923,472
_RS19900
SGL_RS19905: SGL_RS19905 - hypothetical protein, at 1,923,625 to 1,923,897
_RS19905
Position (kb) 1922
1923
1924 Strain fitness (log2 ratio) -1
0
1
2 at 1922.086 kb on + strand at 1922.152 kb on + strand at 1922.167 kb on + strand at 1922.592 kb on + strand, within SGL_RS10955 at 1922.700 kb on - strand at 1922.876 kb on - strand, within SGL_RS10960 at 1922.981 kb on + strand, within SGL_RS10960 at 1923.189 kb on + strand at 1923.434 kb on + strand, within SGL_RS19900 at 1923.577 kb on + strand at 1923.594 kb on - strand at 1923.595 kb on - strand at 1923.922 kb on + strand at 1924.024 kb on - strand at 1924.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,922,086 + -0.3 1,922,152 + -0.2 1,922,167 + -1.3 1,922,592 + SGL_RS10955 0.76 -0.0 1,922,700 - +0.5 1,922,876 - SGL_RS10960 0.26 +0.3 1,922,981 + SGL_RS10960 0.59 +0.8 1,923,189 + -0.2 1,923,434 + SGL_RS19900 0.87 +0.9 1,923,577 + +1.0 1,923,594 - +2.5 1,923,595 - -0.2 1,923,922 + -0.4 1,924,024 - +0.5 1,924,114 - -1.4
Or see this region's nucleotide sequence