Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08945 and SGL_RS08950 are separated by 12 nucleotides SGL_RS08950 and SGL_RS08955 are separated by 66 nucleotides SGL_RS08955 and SGL_RS08960 are separated by 234 nucleotides SGL_RS08960 and SGL_RS19340 are separated by 58 nucleotides
SGL_RS08945: SGL_RS08945 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 1,514,130 to 1,515,338
_RS08945
SGL_RS08950: SGL_RS08950 - DUF2555 domain-containing protein, at 1,515,351 to 1,515,602
_RS08950
SGL_RS08955: SGL_RS08955 - alpha/beta hydrolase, at 1,515,669 to 1,516,424
_RS08955
SGL_RS08960: SGL_RS08960 - flavodoxin FldA, at 1,516,659 to 1,517,171
_RS08960
SGL_RS19340: SGL_RS19340 - hypothetical protein, at 1,517,230 to 1,517,409
_RS19340
Position (kb)
1515
1516
1517 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1515.396 kb on - strand, within SGL_RS08950 at 1515.421 kb on - strand, within SGL_RS08950 at 1515.564 kb on + strand, within SGL_RS08950 at 1515.611 kb on + strand at 1515.639 kb on - strand at 1515.651 kb on + strand at 1515.659 kb on - strand at 1515.669 kb on - strand at 1515.795 kb on - strand, within SGL_RS08955 at 1515.896 kb on - strand, within SGL_RS08955 at 1516.109 kb on + strand, within SGL_RS08955 at 1516.116 kb on - strand, within SGL_RS08955 at 1516.125 kb on + strand, within SGL_RS08955 at 1516.280 kb on - strand, within SGL_RS08955 at 1517.234 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,515,396 - SGL_RS08950 0.18 -0.8 1,515,421 - SGL_RS08950 0.28 +1.3 1,515,564 + SGL_RS08950 0.85 +0.5 1,515,611 + -1.3 1,515,639 - -0.6 1,515,651 + -1.9 1,515,659 - -0.5 1,515,669 - -0.2 1,515,795 - SGL_RS08955 0.17 +0.5 1,515,896 - SGL_RS08955 0.30 +2.2 1,516,109 + SGL_RS08955 0.58 +1.8 1,516,116 - SGL_RS08955 0.59 +0.4 1,516,125 + SGL_RS08955 0.60 +1.8 1,516,280 - SGL_RS08955 0.81 +1.0 1,517,234 + -1.4
Or see this region's nucleotide sequence