Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06865 and SGL_RS06870 are separated by 193 nucleotides SGL_RS06870 and SGL_RS06875 are separated by 37 nucleotides SGL_RS06875 and SGL_RS06880 are separated by 167 nucleotides SGL_RS06880 and SGL_RS06885 are separated by 56 nucleotides
SGL_RS06865: SGL_RS06865 - isoleucine--tRNA ligase, at 1,062,238 to 1,065,204
_RS06865
SGL_RS06870: SGL_RS06870 - hypothetical protein, at 1,065,398 to 1,065,622
_RS06870
SGL_RS06875: SGL_RS06875 - DNA polymerase III subunit gamma/tau, at 1,065,660 to 1,069,010
_RS06875
SGL_RS06880: SGL_RS06880 - VOC family protein, at 1,069,178 to 1,069,609
_RS06880
SGL_RS06885: SGL_RS06885 - c-type cytochrome, at 1,069,666 to 1,071,702
_RS06885
Position (kb)
1065
1066
1067
1068
1069
1070 Strain fitness (log2 ratio)
-1
0
1 at 1065.285 kb on + strand at 1065.319 kb on - strand at 1065.348 kb on - strand at 1069.146 kb on + strand at 1069.261 kb on + strand, within SGL_RS06880 at 1069.261 kb on + strand, within SGL_RS06880 at 1069.363 kb on + strand, within SGL_RS06880 at 1069.531 kb on - strand, within SGL_RS06880 at 1069.799 kb on + strand at 1069.846 kb on + strand at 1069.846 kb on + strand at 1069.985 kb on + strand, within SGL_RS06885
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,065,285 + +0.4 1,065,319 - -0.3 1,065,348 - -0.1 1,069,146 + +1.5 1,069,261 + SGL_RS06880 0.19 -0.6 1,069,261 + SGL_RS06880 0.19 -0.3 1,069,363 + SGL_RS06880 0.43 +0.0 1,069,531 - SGL_RS06880 0.82 +0.0 1,069,799 + -0.0 1,069,846 + +1.2 1,069,846 + -1.4 1,069,985 + SGL_RS06885 0.16 -1.4
Or see this region's nucleotide sequence