Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18640 and SGL_RS02330 are separated by 262 nucleotides SGL_RS02330 and SGL_RS02335 overlap by 36 nucleotides SGL_RS02335 and SGL_RS02340 are separated by 20 nucleotides
SGL_RS18640: SGL_RS18640 - transposase, at 106,905 to 107,208
_RS18640
SGL_RS02330: SGL_RS02330 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 107,471 to 108,325
_RS02330
SGL_RS02335: SGL_RS02335 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 108,290 to 109,201
_RS02335
SGL_RS02340: SGL_RS02340 - hypothetical protein, at 109,222 to 110,313
_RS02340
Position (kb) 107
108
109 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 106.496 kb on + strand at 107.050 kb on + strand, within SGL_RS18640 at 107.112 kb on - strand, within SGL_RS18640 at 107.275 kb on - strand at 107.328 kb on + strand at 107.550 kb on + strand at 107.558 kb on - strand, within SGL_RS02330 at 107.711 kb on - strand, within SGL_RS02330 at 107.782 kb on + strand, within SGL_RS02330 at 107.822 kb on + strand, within SGL_RS02330 at 107.855 kb on - strand, within SGL_RS02330 at 107.933 kb on - strand, within SGL_RS02330 at 108.158 kb on - strand, within SGL_RS02330 at 108.542 kb on + strand, within SGL_RS02335 at 108.544 kb on - strand, within SGL_RS02335 at 108.591 kb on + strand, within SGL_RS02335 at 108.599 kb on - strand, within SGL_RS02335 at 108.599 kb on - strand, within SGL_RS02335 at 108.599 kb on - strand, within SGL_RS02335 at 108.654 kb on + strand, within SGL_RS02335 at 108.655 kb on - strand, within SGL_RS02335 at 108.672 kb on - strand, within SGL_RS02335 at 108.780 kb on - strand, within SGL_RS02335 at 108.965 kb on + strand, within SGL_RS02335 at 108.973 kb on - strand, within SGL_RS02335 at 108.973 kb on - strand, within SGL_RS02335 at 108.973 kb on - strand, within SGL_RS02335 at 108.978 kb on - strand, within SGL_RS02335 at 108.978 kb on - strand, within SGL_RS02335 at 108.983 kb on - strand, within SGL_RS02335 at 109.001 kb on - strand, within SGL_RS02335 at 109.043 kb on + strand, within SGL_RS02335 at 109.051 kb on - strand, within SGL_RS02335 at 109.061 kb on + strand, within SGL_RS02335 at 109.144 kb on + strand at 109.253 kb on + strand at 109.278 kb on + strand at 109.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 106,496 + +1.4 107,050 + SGL_RS18640 0.48 +1.5 107,112 - SGL_RS18640 0.68 +0.0 107,275 - +0.6 107,328 + -0.3 107,550 + -3.2 107,558 - SGL_RS02330 0.10 -2.3 107,711 - SGL_RS02330 0.28 -4.2 107,782 + SGL_RS02330 0.36 -0.3 107,822 + SGL_RS02330 0.41 +1.4 107,855 - SGL_RS02330 0.45 -3.5 107,933 - SGL_RS02330 0.54 -2.8 108,158 - SGL_RS02330 0.80 -2.0 108,542 + SGL_RS02335 0.28 +1.3 108,544 - SGL_RS02335 0.28 +0.0 108,591 + SGL_RS02335 0.33 -0.5 108,599 - SGL_RS02335 0.34 -5.8 108,599 - SGL_RS02335 0.34 -3.2 108,599 - SGL_RS02335 0.34 +0.9 108,654 + SGL_RS02335 0.40 +0.3 108,655 - SGL_RS02335 0.40 +0.2 108,672 - SGL_RS02335 0.42 +1.3 108,780 - SGL_RS02335 0.54 +0.5 108,965 + SGL_RS02335 0.74 +0.8 108,973 - SGL_RS02335 0.75 +2.6 108,973 - SGL_RS02335 0.75 +2.3 108,973 - SGL_RS02335 0.75 +0.8 108,978 - SGL_RS02335 0.75 +3.6 108,978 - SGL_RS02335 0.75 -0.1 108,983 - SGL_RS02335 0.76 +2.1 109,001 - SGL_RS02335 0.78 +0.6 109,043 + SGL_RS02335 0.83 +0.1 109,051 - SGL_RS02335 0.83 -1.1 109,061 + SGL_RS02335 0.85 +0.5 109,144 + +0.4 109,253 + -0.5 109,278 + +0.9 109,280 + +0.7
Or see this region's nucleotide sequence