Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18535 and SGL_RS00770 are separated by 209 nucleotides SGL_RS00770 and SGL_RS00775 are separated by 29 nucleotides SGL_RS00775 and SGL_RS00780 overlap by 4 nucleotides SGL_RS00780 and SGL_RS19375 are separated by 52 nucleotides SGL_RS19375 and SGL_RS19380 are separated by 45 nucleotides SGL_RS19380 and SGL_RS00785 are separated by 31 nucleotides SGL_RS00785 and SGL_RS00790 are separated by 191 nucleotides
SGL_RS18535: SGL_RS18535 - hypothetical protein, at 29,928 to 30,142
_RS18535
SGL_RS00770: SGL_RS00770 - DUF86 domain-containing protein, at 30,352 to 30,669
_RS00770
SGL_RS00775: SGL_RS00775 - DUF5615 family PIN-like protein, at 30,699 to 31,040
_RS00775
SGL_RS00780: SGL_RS00780 - DUF433 domain-containing protein, at 31,037 to 31,369
_RS00780
SGL_RS19375: SGL_RS19375 - hypothetical protein, at 31,422 to 31,595
_RS19375
SGL_RS19380: SGL_RS19380 - hypothetical protein, at 31,641 to 31,790
_RS19380
SGL_RS00785: SGL_RS00785 - hypothetical protein, at 31,822 to 32,097
_RS00785
SGL_RS00790: SGL_RS00790 - hypothetical protein, at 32,289 to 32,483
_RS00790
Position (kb) 31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 30.063 kb on - strand, within SGL_RS18535 at 30.147 kb on - strand at 30.157 kb on - strand at 30.232 kb on + strand at 30.232 kb on + strand at 30.240 kb on - strand at 30.261 kb on - strand at 30.284 kb on - strand at 30.842 kb on - strand, within SGL_RS00775 at 30.979 kb on + strand, within SGL_RS00775 at 30.991 kb on + strand, within SGL_RS00775 at 31.064 kb on + strand at 31.298 kb on + strand, within SGL_RS00780 at 31.298 kb on + strand, within SGL_RS00780 at 31.378 kb on - strand at 31.378 kb on - strand at 31.427 kb on - strand at 31.453 kb on - strand, within SGL_RS19375 at 31.512 kb on - strand, within SGL_RS19375 at 31.536 kb on - strand, within SGL_RS19375 at 31.669 kb on + strand, within SGL_RS19380 at 31.754 kb on + strand, within SGL_RS19380 at 31.913 kb on - strand, within SGL_RS00785 at 31.920 kb on + strand, within SGL_RS00785 at 32.008 kb on + strand, within SGL_RS00785 at 32.039 kb on - strand, within SGL_RS00785
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 30,063 - SGL_RS18535 0.63 +0.0 30,147 - -1.1 30,157 - -0.7 30,232 + +0.4 30,232 + +0.1 30,240 - +0.6 30,261 - -0.3 30,284 - -0.1 30,842 - SGL_RS00775 0.42 -0.8 30,979 + SGL_RS00775 0.82 +0.6 30,991 + SGL_RS00775 0.85 -0.5 31,064 + -0.9 31,298 + SGL_RS00780 0.78 -2.1 31,298 + SGL_RS00780 0.78 -3.2 31,378 - -1.6 31,378 - +0.1 31,427 - +0.2 31,453 - SGL_RS19375 0.18 -0.5 31,512 - SGL_RS19375 0.52 -2.6 31,536 - SGL_RS19375 0.66 +0.4 31,669 + SGL_RS19380 0.19 -2.1 31,754 + SGL_RS19380 0.75 -1.1 31,913 - SGL_RS00785 0.33 -0.6 31,920 + SGL_RS00785 0.36 -0.6 32,008 + SGL_RS00785 0.67 +0.5 32,039 - SGL_RS00785 0.79 +0.9
Or see this region's nucleotide sequence