Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16870 and SGL_RS16875 are separated by 106 nucleotides SGL_RS16875 and SGL_RS16880 are separated by 4 nucleotides SGL_RS16880 and SGL_RS20195 are separated by 136 nucleotides SGL_RS20195 and SGL_RS16890 are separated by 164 nucleotides
SGL_RS16870: SGL_RS16870 - molecular chaperone DnaJ, at 3,237,125 to 3,238,258
_RS16870
SGL_RS16875: SGL_RS16875 - crossover junction endodeoxyribonuclease RuvC, at 3,238,365 to 3,238,868
_RS16875
SGL_RS16880: SGL_RS16880 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 3,238,873 to 3,239,748
_RS16880
SGL_RS20195: SGL_RS20195 - hypothetical protein, at 3,239,885 to 3,240,016
_RS20195
SGL_RS16890: SGL_RS16890 - indole-3-glycerol phosphate synthase TrpC, at 3,240,181 to 3,241,068
_RS16890
Position (kb) 3238
3239
3240 Strain fitness (log2 ratio) -2
-1
0
1 at 3238.982 kb on + strand, within SGL_RS16880 at 3239.280 kb on - strand, within SGL_RS16880 at 3239.346 kb on - strand, within SGL_RS16880 at 3239.476 kb on - strand, within SGL_RS16880 at 3239.667 kb on - strand at 3239.815 kb on - strand at 3239.853 kb on - strand at 3239.858 kb on + strand at 3240.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 3,238,982 + SGL_RS16880 0.12 -0.8 3,239,280 - SGL_RS16880 0.46 -2.1 3,239,346 - SGL_RS16880 0.54 -1.6 3,239,476 - SGL_RS16880 0.69 -1.0 3,239,667 - -1.1 3,239,815 - +0.3 3,239,853 - -0.5 3,239,858 + +0.1 3,240,063 - -0.1
Or see this region's nucleotide sequence