Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05645 and SGL_RS05650 are separated by 82 nucleotides SGL_RS05650 and SGL_RS05655 are separated by 40 nucleotides SGL_RS05655 and SGL_RS05660 are separated by 156 nucleotides
SGL_RS05645: SGL_RS05645 - hypothetical protein, at 815,703 to 816,215
_RS05645
SGL_RS05650: SGL_RS05650 - Uma2 family endonuclease, at 816,298 to 816,942
_RS05650
SGL_RS05655: SGL_RS05655 - TPM domain-containing protein, at 816,983 to 817,810
_RS05655
SGL_RS05660: SGL_RS05660 - transketolase, at 817,967 to 819,979
_RS05660
Position (kb) 816
817
818 Strain fitness (log2 ratio) -2
-1
0
1 at 816.016 kb on - strand, within SGL_RS05645 at 816.062 kb on - strand, within SGL_RS05645 at 816.062 kb on - strand, within SGL_RS05645 at 816.071 kb on + strand, within SGL_RS05645 at 816.099 kb on + strand, within SGL_RS05645 at 816.187 kb on + strand at 816.687 kb on + strand, within SGL_RS05650 at 816.722 kb on + strand, within SGL_RS05650 at 816.752 kb on + strand, within SGL_RS05650 at 816.790 kb on + strand, within SGL_RS05650 at 816.963 kb on + strand at 817.263 kb on + strand, within SGL_RS05655 at 817.263 kb on + strand, within SGL_RS05655 at 817.263 kb on + strand, within SGL_RS05655 at 817.263 kb on + strand, within SGL_RS05655 at 817.296 kb on + strand, within SGL_RS05655 at 817.576 kb on - strand, within SGL_RS05655 at 817.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 816,016 - SGL_RS05645 0.61 -0.2 816,062 - SGL_RS05645 0.70 -0.1 816,062 - SGL_RS05645 0.70 +0.3 816,071 + SGL_RS05645 0.72 -0.3 816,099 + SGL_RS05645 0.77 -0.3 816,187 + +0.4 816,687 + SGL_RS05650 0.60 -0.1 816,722 + SGL_RS05650 0.66 +0.2 816,752 + SGL_RS05650 0.70 +0.0 816,790 + SGL_RS05650 0.76 +0.2 816,963 + +0.0 817,263 + SGL_RS05655 0.34 -2.5 817,263 + SGL_RS05655 0.34 -1.3 817,263 + SGL_RS05655 0.34 -0.2 817,263 + SGL_RS05655 0.34 -0.7 817,296 + SGL_RS05655 0.38 -1.2 817,576 - SGL_RS05655 0.72 -0.1 817,865 - +0.1
Or see this region's nucleotide sequence